Advanced Materials Research Vols. 781-784

Abstract: The infrared spectra of serum from cancer patients can be measured using Fourier Transform Infrared Spectroscopy (FTIR). The changes of infrared absorption peak position in phosphor diester groups from cancer cells using OriginPro8.1 software indicates that nucleic acids contents in the cancer serum rise above normal serum levels and that the binding force between hydrogen bonds is strengthened. Calculation of the relative concentration of serum glycogen shows that glycogen concentration in cancer affected patient serum is lower than that of healthy patient serum. Infrared absorption analysis demonstrates that intermolecular hydrogen bonds between C―O groups are largely destroyed in cancer patient serum.

Abstract: In this paper, the condition of aspergillus niger and the bacillus subtilis mixing fermentation to produce soybean peptides was studied. The results indicated that the best fermentation condition of the aspergillus niger and the bacillus subtilis mixing fermentation to produce soybean peptides is that: the initial pH of the culture medium is 8.0, the proportion of mixture strains (aspergillus niger vs bacillus subtilis) is 2 to 1,the fermentation temperature is 30°C and the fermentation time is 80 hours. In this condition the degree of hydrolysis of the fermentation bean pulp is 36.5%.

Abstract: Marine adhesion organism includes biological mucosa such as marine bacteria, diatom, etc. and large adhesion organism such as mussel, barnacle, etc. Researches and analysis on adhesion mechanism of adhesion organism show that adhesion marine bacteria in biological mucosa will secrete protein-containing Polysaccharide polymer (PAVE) which can adhere to all kinds of surfaces. The reason is that in these secretions there is 3, 4-dihydroxyphenylalanine (DOPA) which is very viscous. Analysis on mussel, a large adhesion organism, shows that it is of super viscosity, which may result from its special molecular structure and the interaction way with substrates, and interstrand crosslink mediated by DOPA. DOPA plays an important role in this process. For marine bacteria and mussel, their viscosity is correlated with the generation and cross-linking of DOPA. On one hand, DOPA can enhance the viscosity of adhesion organism; on the other hand, it can improve the internal cohesion through cross-linking.

Abstract: Saccharomyces cerevisiae R40 and Pachysolen tannophilus P01 were used as the parental strain to construct an engineering strain capable of co-fermenting pentose and hexose by protoplast fusion. A fusant F202 was obtained through inactivating parental protoplasts, screening with YPX solid medium and high glucose liquid medium, ethanol production capacity detecting and identification with PCR-SSR technique. Subsequently, the fermentation performance and genetic stability of F202 was studied. The maximum ethanol production capacity from glucose was 1.47 ml/100 ml with a sugar and alcohol conversion rate 47% which was 11% higher than the parental strain P01. By fermenting xylose the ethanol concentration could achieve to 0.58 ml/100 ml with a sugar and alcohol conversion rate 12%. An ethanol concentration of 1.2 ml/100 ml was obtained by fermenting the mixture of xylose and glucose (mass ratio 1:2). Moreover, no decrease in ethanol yield after 8 generations propagation suggested fustant 202 possessed good genetic stability.

Abstract: In order to purify the platycodins from Platycodon grandiflorum, AB-8 macroporous resin was used to test. Based on single factor experiments, orthogonal test was used to optimum the purification conditions. Adsorption pH, adsorption temperature, adsorption time and platycodins concentration were as factors and adsorption capacity was as index. The results were as follows: adsorption pH 6.0, adsorption temperature 40°C, adsorption tim110min, platycodins concentration 2.0mg/mL were the optimization conditions.The adsorption quantity reached at 39.1mg/g. So AB-8 macroporous resin was a suitable resin for purify the platycodins from Platycodon grandiflorum.

Abstract: Fermentation of xylanase produced by Trichoderma reesei was conducted in 250mL shake flasks. Several fermentation conditions was investigated that affected production of xylanase by Trichod erma reesei, including inoculum age, inoculum size, medium volume, shear stress, temperature, shaking speed and initial pH. Results showed that the the optimum culture conditions were as follows: inoculum age 45h, inoculum size 10%,medium volume 50mL/250mL, shaking speed 200rpm and initial pH4.0(natural). The maximum xylanase activity under each condition was occurred at 104h and the maximal activities can reach 1425.27U/mL.

Abstract: For finding microbes of degradation phosphine (PH₃) in CO, a low concentration PH₃ gas in CO was used to induce domestication the microorganism from anaerobic tank of sewage treatment plant. The influence of domestication process was researched in detail, and the microbial growth and performance of dephosphorization domesticated was inspected. The results shown that PH₃ concentration, pH, and replaced water have a greatly influence for the process. A group dephosphorization bacterium which have a characteristic of fast growth and reproduction and degradation PH₃ in CO to phosphate was acquired after domesticated the bacteria from anaerobic tank more than 11d. Appropriate conditions of dephosphorization bacteria to degradation PH₃ are pH=4.5-5.5, 30°C. The phosphine conversion rate was 68.59% and 65.32% for 24h respectively to the concentration of 12 ppm or7ppm PH₃ in CO and the 2/3 of products was phosphate primarily. But the aerobic tank bacteria cannot be domesticated.

Abstract: A simple and clean way of extraction of natural essence from green tea leaf by supercritical CO₂ was developed. The optimum extraction conditions to produce the high-quailty natural essence were found: loading about 100g/L, operating at 5000 psi and 50 °C, and separation kettle temperature 75 °C, static extraction three times with 40 min every time, the yield of natural essence was 0.5%. The product was analyzed by gas chromatography-mass spectrometry (GC-MS) show that dimethylsulfide was the characteristic aroma components of the natural essence with more than 50 kinds of alcohols, ketones, aldehydes, ethers and esters etc.. There were only little other biochemical composition of raw material and 0.4% caffeine in the product detected by high-performance liquid chromatography (HPLC), and the biochemical composition in the raw material were almost no loss before and after extracted by supercritical CO₂, it indicated that the specificity of the method for extraction tea natural essence is very strong, and propitious to improve the secondary utilization of the tea raw materials.

Abstract: Taking the degree of hydrolysis (DH) as the index, the optimal hydrolysis process for the protein from black bean was explored by response surface methodology (RSM) to prepare polypeptides. Four proteases were used to hydrolyze the black bean protein and determine the DH of black bean protein. The results indicated that the optimal reaction systems were as follows: The black bean protein solution was treated with the alkaline protease hydrolysis firstly and then the trypsin hydrolysis was used in order to improve the DH. The DH of protein was increased to 35.64% and the yield of peptides production was higher than using only alcalase.

Abstract: Abelmoschus Manihot (L.) Medic flowers are traditionally used for food and medicinal materials. With the aim of acquiring the best method of isolating hyperoside from total flavone in A. Manihot flowers, this study investigated the effects of different eluted factors on separating hyperoside in total flavone of crude extracts. Gradient elution was used to separate the total flavone of ethanol extraction. The eluates were isolated by silica gel thin-layer chromatography (TLC) and identified by HPLC. The results showed that the elution components (chloroform: methanol = 50: 50) isolated by TLC (developing agent ethyl acetate: formic acid: water = 8:3:1) presented a clear light yellow stripe which was identified as hyperoside by HPLC.