Advanced Materials Research Vols. 781-784

Abstract: The main chemical compounds in pyrethrum flower extract obtained by supercritical fluid extraction (SFE) were identified by GC-MS. Peak area normalization was used to determine relative percentage content of the compounds. The results showed that the main chemical compounds in pyrethrum flower extract were β-farnesene, β-cubebene, ethyl palmitate and ethyl linoleate, besides six pesticidal active compounds of pyrethrins that were cinerin I, jasmin I, pyrethrin I, cinerinII, jasminIIand pyrethrinII.

Abstract: High-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of Ginkgo flavonoids from the Ginkgo biloba L. leaf extract (GBE) with a two-phase solvent system composed of n-hexaneethyl acetatemethanolwater at an optimized volume ratio of 4:6:5:5(v/v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. The preparative HSCCC separation was performed on 200 mg of GBE yielding pure Quercetin (22mg), Kaempferol (15mg) and Isorhamnetin (4mg) at purities of 96.6%, 92.3% and 93.6%, respectively, as determined by high performance liquid chromatography (HPLC). HSCCC is a powerful technique for isolation and separation of chemical composition from GBE.

Abstract: To investigate the impact of AGEs with different concentrations and different times on the expression of UⅡ and GPR14 mRNA in MC. Methods In vitro rat mesangial cells, by adding different concentrations of AGEs(final concentration0、25、50、100和200 mg/L)，37°C incubated for 24 h,AGE100 mg/L groups were cultured 0、2、8、16 and 24 h separately，established the control groups respectively, collecting MC,extracted total cellular RNA for RT-PCR reaction and detected the expression of UⅡ and GPR14 mRNA in MC. Results The expression of UⅡ and GPR14 mRNA in MC were increased with the change of AGEs concentrations from low to high.Compared with AGEs 0 mg / L group, the expression of UⅡ and GPR14 mRNA in AGEs50, 100, and 200 mg / L groups were significantly higher (P<0.05); In AGEs100mg / L groups, the expression of UⅡ and GPR14 mRNA in MC were increased with the time extended, compared with AGEs 0h group, the expression of UⅡ and GPR14 mRNA in AGEs8, 16, and 24h groups were significantly higher (P<0.05); There were no change in control groups（P＞0.05）. Conclusion AGEs can significantly increase the expression of UⅡ and GPR14 mRNA in MC.

Abstract: This paper lists the results of the study of sunflower processing renewable wastes fruit walls (husk) sampled in various Russian regions for the purpose of their use in making various chemical matters and materials with useful properties. Sorption properties of various sunflower fruit walls with reference to Sakhalin crude oil compared to similar wastes of buckwheat and rice and inorganic sorbent (vermiculite). The content and structure of inorganic components of husk samples was studied depending on the sort. Water-soluble materials extracted from fruit walls in different conditions was studied. The content and structure of water/acid/alkaline extraction polysaccharides was found. The ability to use liquid and dry sunflower husk extracts as steel corrosion inhibitors was shown. Lipids contained in sunflower making wastes were described. It was shown that they contain mostly monogalactosyldiacylglycerides and phospholipids which are important bioactive matters.

Abstract: To separate and enrich morin, a molecularly imprinted polymer (MIP) was synthesized on chitosan by surface molecular imprinting technique and characterized with Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The results showed that MIP possessed better selectivity and recognition for morin in a mixture than that of non-imprinted polymer (NIP). The saturated adsorption amount of MIP was 8.0 mg/g, which was 4-fold than that of NIP, and the recovery in the elution step was 94.87% and 10.97% for MIP and NIP, respectively. These findings indicate MIP could realize the separation and pre-concentration of morin in real sample and may be used for solid phase extraction (MIP-SPE) protocol.

Abstract: The 95% ethanol extracts of mangifera indica leaf was sequentially extracted with petroleum ether, ethyl acetate, n-butyl alcohol and water. Antibacterial activity and minimal inhibitory concentration (MIC) of the four different extraction against Staphylococlus aureus, Escherichia coli, Bacillus subitlis and Saccaromyces cerevisiae were stuied with filter paper method in this paper. Each of the four extracts showed different antibacterial activity: The water extract is the strongest one; l-butanol extract takes second place; Ethyl acetate and petroleum ether extract is the third. MIC of aqueous extract with the strongest inhibitory effect on Staphylococlus aureus, Escherichia coli and Saccaromyces cerevisiae is 0.0125 g/mL, 0.0125 g/mL, and 0.025 g/mL. Moreover, the aqueous extract showed powerful antibacterial activity against all of four bacteria.

Abstract: The pigment was isolated from fructus ligustri lucidi. The pigment belonges to flavonoid by color reaction and absorption spectrum analysis. The color scale was tested, with emphasis on the effects of pH, temperatures, sunlight, oxidizing agent, reducing agent, salt, vitamin C, preservative and sucrose on its stability. Results show that the color scale of yellow pigment from fructus ligustri lucidi is 52.38. It is stable under acidic condition and degraded under alkaline condition. It has good heat and light resistance. The pigment has better endurance capability against oxidizing agent but reducing agent. The effects of salt and sucrose are relatively small.Vitamin C has a certain increment on the color, but preservative would degrade the pigment. The study shows that yellow pigment has good stability and supports the application in the food industry.

Abstract: Far-UV circular dichroism (CD) spectroscopy was used to study the conformation of wheat gluten protein treatmented by dynamic high pressure microfluidization (DHPM), acid treatment and its comprehensive treatment in two solvents. The results showed, the secondary structure of control sample are mainly consist of α-helix and random-coil in phosphate-buffered saline (PBS) and phosphate buffered solution with SDS(SDS), the secondary structure of control sample are mainly consist of β-Sheet and random-coil. The CD data also showed that SDS interacts with the gluten protein and modifies the protein conformation, which switched the conformation from α-helix and β-Turn to β-sheet and random-coil. However, the CD analysis also indicated that some of the ordered structures of α-helix, β-Turn and β-sheet were destroyed and converted random-coil coped with acid in two solvents, in other words, the acid treatment can directed change the secondary structure. Furthermore, the effect of comprehensive treatment (DHPM plus acid) is not equal to the simple sum of the individual treatment effect.

Abstract: In this paper,tannase was immobilized by the method of the pre cross-linking,embedding and re cross-linking.The orthogonal design is carried out on the basis of single factor,by which the optimum conditions of the immobilized tannase was selected out ,the optimum pre cross-linking and re cross-linking concentration of glutaraldehyde is 0.3%(v/v )and 0.4%(v/v),respectively,the pre cross-linking temperature is 30°C and the re cross-linking time is 1.0h.in addition,the optimum catalytic temperature is 50°C and the optimum catalytic pH is 5.0,and compered with the free tannase ,the temperature and the pH stability of immobilized tannase improved markedly.

Abstract: Lycopene is gaining more and more attention because of its physiological activities, and it is widely used in food additives, cosmetics and other industries. For lycopene production by microorganism, lycopene-producing strains were screened from oil-immersed soil. A strain of S-1 was successfully screened by analysing the cells extract using thin-layer chromatography (TLC). On the basis of morphological characteristics and sequence analysis of 26S rDNA of strain S-1, the strain S-1 was identified as Rhodotorula mucilaginosa. The lycopene production by Rhodotorula mucilaginosa in fermentation broth reached 7.4 mg/L by 108 h.