Advanced Materials Research Vols. 781-784

Abstract: In this paper, paeonol was studied systemtically. At first, the affinity interaction of paeonol with bovine serum albumin (BSA) was investigated through capillary electrophoresis approach. The association constant of paeonol and BSA was obtained by the vacancy affinity capillary electrophoresis method (VACE). The second, the contents of paeonol were determinede in liuweidihuang pill. In this paper, the new studied method and basic data were offered for further investigating the pharmacology and drug efficacy of paeonol. Meanwhile, the method described would be particularly useful in the quality control of traditional Chinese medicines-liuweidihuang pill.

Abstract: This paper is mainly to investigate the stability of synthetic folic acid under different conditions, including pH, temperature, light, and the presence of antioxidants. The results show that folic acid gives a good stability in the buffer of pH 8.05~10.40, whose reservation ratio can reach more than 93.1% in 72 h, while the preservation ratio of folic acid reduce to 23% in the buffer of pH1.95~6.40. Moreover, folic acid has a good stability in the buffer of pH 5.39~10.40, after water bath at 100°C for 2 h. Folic acid exhibits a good stability under the condition of weak illumination intensity. Increasing illumination intensity promotes a decrease in FA concentration. The combination of antioxidants Vitamin C (VC) and sodium citrate displays a significant effect on protecting folic acid from oxidation.

Abstract: Benzimidazole compounds are known to possess varied biological activities and so far various types of benzimidazole drugs have extensively been used in clinic. Benzimidazole compounds as a antibacterial agents has been a quite rapidly developing and is gradually becoming a relatively independent scientific area. In this paper we presented the recent advances of benzimidazole compounds as antibacterial agents. The perspective of the foreseeable future and potential application of benzimidazole as antibacterial agents are also presented.

Abstract: A direct and novel ultraviolet spectrophotometry method for analysis of complex samples was researched and developed with the use of chemometrics, using the Miconazole nitrate, econazole nitrate,acetic acid chloride as example samples. The spectra (range: λ=240~280 nm were processed using Partial least squares (PLS).The ultraviolet spectrophotometry data of the samples were also used to evaluate the samples’ quantitative composition.It was found that the samples were clearly discriminated.A PLS model was developed for simultaneous analysis of the three analytes . The satisfactory prediction results for synthetic samples were obtained by Partial least squares (PLS) as calibration methods (relative prediction error RPET =4.23%).

Abstract: In this study, our objective was to prepare levalbuterol hydrochloride drug-resin and investigate its characteristics. This work is basis for the study of the next levalbuterol hydrochloride sustained-release suspensions. The drug-resin was prepared by bath method. The effect of factors , including resin types, drug concentration, speed, temperatures and pH on evaluation index, was evaluate by Drug utilization rate and the resin loading. Then the Box-Behnken Design-response surface method was used to optimize the preparation. The drug content determined by UV method and drug-resin were characterize by IR and DSC．The best formulation of levalbuterol hydrochloride drug-resin was used the bath method IRP69 resin at 30°C. The drug concentration was 1.8 mg•ml^-1, and the rotate speed was 250 rpm, the pH was 5.8. The preparation of levalbuterol hydrochloride drug-resin method is simple and feasible, and the drug combined with the resin by the chemisorption of ionic bond.

Abstract: Aurones belong to a class of heterocyclic flavonoids which contains a benzofuran element associated with a benzylidene linked in position 2. Aurones possess a wide range of pharmacological activities and biological activities, such as antitumor, antifungal, phytoalexin and so on. A novel series of 2-ayl-yl (5-methacrylate) aurone analogues were synthesized in six steps with the overall yield of 11%-13% and characterized by ¹H NMR. Among the key intermediates and target compounds, 2-(2-furan-ylmethylene)-5-methacrylate-benzofuran-3(2H)-one (7a) and 2-(2-thienyl-ylmethylene)-5-methacrylate-benzofuran-3(2H)-one (7b) have never been reported before. Primary biological activities evaluation showed that 7a exhibited good inhibitory activities against K562 with an IC₅₀ of 2.18 μM and against HepG2 with an IC₅₀ of 3.95μM.

Abstract: Indolone is a kind of natural product which was widely existed in the animal bodies and plants, which possess varieties of biological activities, such as anti-bacterial, anti-tumor, anti-aging and anti-anxiety activities. As we all known that many sprio compounds which containing hetero atom such as O, N, S have good biological activities. In this paper, a series of spiro indolone derivatives was design with the combination of indolone and sprio scaffold, 6 novel target compounds were synthesized in 3 or 4 steps in 6%-17% overall yield, which were characterized by ¹H NMR and their biological activities on tumor cells growth inhibition on human hepatoma cell HePG2 and human leukemia cell K562 by MTT method were evaluated.

Abstract: In order to generate the specific antibody of small molecular hapten-ciprofloxacin for detecting the Ciprofloxacin residues in edible animal tissues. A modified Carbodiimide coupling method was developed to obtain the immunogenic conjugates through coupling ciprofloxacin with bovine serum albumin in five different proportions. The conjugates were characterized and verified by electrophoresis,the trinitro-benzene-sulfonic acid (TNBS) method and ultraviolet spectra scanning. A quantitative analysis of the density of ciprofloxacin-bovine serum albumin conjugates suggested that a high conjugate density 30:1 could be obtained in the optimized conditions. By immunizing the mice, the antibody showed high sensitivity toward ciprofloxacin with an IC₅₀ of 4.69 ng/ml. This study confirmed a practical approach for the ciprofloxacin-bovine serum albumin conjugates, through which the high potent antisera specific for ciprofloxacin hapten can be produced for immunoassay.

Abstract: Oviductus rana is a traditional medicinal product of Jilin province, China. The objective of this study was to observe the effects of the oviductus rana hydrolysate on the immune functions of immunosuppressed mice. For the purpose, this product was hydrolyzed by the combination of papain and compound proteases for which the optimum conditions of hydrolysis were also achieved. The optimum hydrolysis conditions found in this study were: composite enzyme concentration 4%; temperature 50 °C; time 4 h. The results showed that the coefficients of spleen and thymus were increased in the dose groups (P<0.05) whom oviductus rana hydrolysates were given in different concentrations as compared to the model group (without oviductus rana hydrolysate). These parameters increased as a function of concentration of oviductus rana hydrolysate. Carbon particle clearance index were also higher in the dose groups than that of model group and control. This index was the highest in the high dose group and increased as a function of dose of oviductus rana hydrolysate. The ability of macrophage as well as the delayed type hypersensitivity increased significantly in the dose groups (P<0.01). Meanwhile, the contents of IgM and IgG were also raised (P<0.01) in middle and low dose groups whereas decreased in high dose groups. It is found that oviducts rana hydrolysate had a marked effect on the immunity of immune-suppressed mice.

Abstract: A replication probe vector (pUB380) was constructed for detecting the replicon of LAB plasmid. A cryptic plasmid, pEV105, was isolated from Enterococcus faecium KLDS 6.0718. Multiple restriction endonuclease fragments of pEV105 were separately ligased to pUB380. After a series of subcloning and electrotransformation, the minimal replicon of pEV105 was isolated on a 2.5 kb Pst I- Xbal I fragment. Replicon based on this region followed a theta-type mechanism of replication in Enterococcus faecium KLDS 6.0718. The minimal replicon DNA fragment was sequenced and the result shows that the gene sequence homology has 99.8 % compared with partial sequence of pCIZ2 from Enterococcus faecium L50. Five putative ORFs were concluded by software. The result of alignments indicated two ORFs, repAl05 and repBl05, encoding two putative proteins RepAl05 and RepBl05 of 245 and 178 amino acids respectively. The 2.5 kb fragment of minimal replicon as a stable replicon is feasible for constructing food-grade cloning and expressing vector of lactic acid bacteria.