Extraction of Dissoluble Protein from Antlers by Pulsed Electric Fields

Abstract:

The extraction of dissoluble protein from antlers using high intensity pulsed electric fields (PEF) was investigated, and then, the extract was preliminary separated by ultrafiltration, and tested the antioxidant activity of each part. The effect of extraction rate of protein among different ratio of material and water, field strength, pulse number and times ware investigated in this text. And optimize the test by ternary quadratic regression rotation combination design, the optimum condition was determined as: ratio of material and water1:12, field strength27.03kV/cm, pulse number12,times 2. Under such condition the maximal rate of extraction of dissoluble protein is 27.32%. The three parts of proteins by ultrafiltrated had been confirmed with DPPH, superoxide anion radical scavenging ability.

• Periodical: Advanced Materials Research (Volumes 850-851)
• Pages: 1141-1144
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.850-851.1141
• Online since: December 2013
• Authors: Yong Guang Yin*, Wei Jiang, Wei Jia Liu
• Keywords: Antioxidant Activity, Antlers, Dissoluble Protein, Pulsed Electric Field (PEF)

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