Advanced Materials Research Vols. 884-885

Abstract: Based on splicing by overlapping extension (SOE) polymerase chain reaction (PCR) ,the ag85a and mpb70 were amplified and the fusion gene ag85a-mpb70 were cloned into pMD18-T vector, and then we got the recombinant plasmid pMD-85a-70. pMD-85a-70 and pET28a (+) were digested by BamHI and EcoRI double enzymes. The purified ag85a-mpb70 fusion gene was subcloned into the expression vector pET28a (+),and the prokaryotic expression vector pET-85a-70 was constructed. Plasmid containing pET-85a-70 was transformed into competence Escherichia coli BL21(DE3).The bacterium was induced by isopropyl-β-D-thiogalactopyranoside (IPTG) and analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), approximately 49 kDa fusion protein was observed on the SDS-PAGE. The protein was analyzed by using Western-blotting. The results indicated that Ag85A-MPB70 was of antigenic activity of Mycobacterium bovis. These results could serve as a basis for further studies on the usefulness of the fusion gene and its expression product in the development of novel vaccine against bovine tuberculosis.

Abstract: For the properties of activated sludge studies, three kinds of activated sludge were obtained from continuous hydrogen production reactor. Activated sludge was got under organic loading rate (OLR) of 3, 7 and 25 kgCOD/m³·d condition, respectively. Sedimentation performance and activities of sludge were investigated. When OLR was 3 kgCOD/m³·d, activated sludge showed good sedimentation performance. After 30 minutes sedimentation, the volume of activated sludge in total mixture volume was about 39%. When OLR was 7 and 25kgCOD/m³·d, after 30 minutes sedimentation, the volume of activated sludge in total mixture volume was 80% and 83%, respectively. The increase of biomass is the main reason for increase of sedimentation performance. MLVSS/MLSS of activated sludge was 37.7% and 79.6% under OLR of 3 and 25kgCOD/m³·d condition, therefore, activities of activated sludge was high under high OLR condition. Since sedimentation performance of sludge is high under high OLR condition, hydraulic retention time should controlled carefully in engineering operation.

Abstract: Woody biomass fuel from the biomass power plant was used as the test sample, of which ash content and C, H and O contents were measuredin this paper.To quantitative analysis the correlation between the woody bio-fuel ash contents and ultimate analysisindexes, the multivariate linear regression and error analysis methods were employed. In the developed regressionmodel, the ash content was recognized as the dependent variable and the contents of C, H and O are recognized as independent variables. Themodel result shows that the ash content of woody biomass has the significant negative correlation relationship with the contents of C, H and O elements.Moreover, the model prediction result also indicates that the prediction error would be minor if the ash content and C, H and O contentsare in the appropriate ranges which are defined by the proposed regression model.

Abstract: The article adopted the Jensen - Shannon Divergence analysis method for alpha wave EEG complexity analysis, used to quantify the three different status (Eyes closed, count, idle) degree of coupling between acquisition of EEG time series. The algorithm are used to calculate the statistical complexity of alpha wave EEG signals then T test, the results show that the state of eyes closed and idle under the coupling degree between EEG time series, and the state of eyes closed and counting, counting and daze cases EEG time series have significant differences. Thus JSD algorithm can be used to analyze EEG signals attention, statistical complexity can be used as a measure of brain function parameters and would be applied to the auxiliary clinical brain function evaluation in the future.

Abstract: Thirty seven strains of potassium-solubilizing bacteria were isolated from rhizosphere soil samples of different plants in Shannxi, China. Among them, six strains were selected by their high capacities of dissolving potassium,and then DBD plasma mutation was conducted to them for improving their releasing potassium activity. The results showed that the releasing potassium activities of most of the mutants increased compared with their initial wild-type strains. The potassium-solubilizing activity of the best mutants called VD-4-1 increased to 20.12mg/l. In comparison to its original strain, the ability of releasing potassium of VD-4-1 increased to 1.91 times. Through 16sr RNA sequence detection, VD-4-1 was identified as Enterobacter sp.. It demonstrated that DBD plasma treatment had a high-efficient quality, and it will be a useful method for micro-organisms mutation. Keywords: Potassium-solubilizing bacteria, Ability of releasing potassium, Dielectric barrier discharge (DBD) plasma mutation

Abstract: A simple and inexpensive high performance capillary zone electrophoresis (CZE) was applied to separate and determine three alkaloids in Radix Aconiti Praeparata simultaneously. The optimized conditions for separation were obtained using 20 mmol/L borax buffer (pH 9.0) containing 2 mmol/L beta-cyclodextrin (β-CD) and 20% methanol. Online UV detection was performed at 235 nm. A voltage of 16 kV was carried at 25°C. Injection was performed at 0.5psi for 10 s. The separation and determination were satisfactory and quick.

Abstract: The light stability, wet stability and thermal stability of the controlled-release tablets of sasanquasaponin (SQS)-casein were studied, respectively. The median lethal dose of SQS and the controlled-release tablets of SQS-casein were studied respectively. After strong light illumination the controlled-release tablets of SQS-casein for 5 days and 10 days respectively and placement the controlled-release tablets of SQS-casein for 5 days and 10 days respectively in humidity being 75±5, respectively, the release rates of the controlled-release tablets of SQS-casein meet the requirements of United States Pharmacopoeia, and the controlled-release tablets of of SQS-casein release SQS by slowness and constant in 12h. When humidity were 90±5% , the release rates of the controlled-release tablets of SQS-casein meet the requirements, but the controlled-release tablets of of SQS-casein do not release SQS by constant in 12h. When temperature were 40±2°C and 60±2°C respectively, the release rates of the controlled-release tablets of SQS-casein does not meet the requirements. The anti-high temperature and moistureproof measures are required in production, packaging, transpor-tation and storage of the controlled-release tablets of SQS-casein. The toxicity of the controlled-release tablets of SQS-casein are much lower than that of SQS.

Abstract: We reported a structure-based virtual screening of acompound library derived from 3-acyl-5-hydroxybenzofurans to develop potentialdrugs for treating breast cancer. A library of 160,000 compounds was generatedand screened based on the G-score between ligands and receptor ERα. The topstructures were further analyzed to evaluate the receptor-ligand bindinginteractions. By comparing the binding characteristics and docking scoringvalues to the existing ERα analogues, we determined top 200 compounds aspotential drug candidates for breast cancer.

Abstract: In order to study the ability of Lactobacillus bulgaricus hydrolyzed protein, using casein sodium as substrate, the content of free amino acid and relative ORAC value were used as the index optimization. The conditions including: hydrolysis temperature, cultivation time, Lactobacillus bulgaricus dosage and pH values were investigated by single-factor experiment. In this paper we studied the hydrolysis conditions of Lactobacillus bulgaricus on casein sodium by the Box-Behnken response surface and the optimal hydrolysis temperature, cultivation time, Lactobacillus bulgaricus dosage and pH value were determined to be 43 °C, 36 h, 25 OD/ml, and pH 6.6, respectively. The optimum value of response surface is 857.75 mg/l. In addition, the analysis of relative ORAC value of response surface showed that there is no direct relationship between the degree of protein hydrolysis and radical scavenging activity, namely as extensive hydrolysis could have a negative impact on the antioxidant activity.

Abstract: To identify the intestinal microflora diversity of transgenic sheep and non-transgenic one with overexpression of foreign antiviral gene (VP1 & SB-SW), DNA of total bacteria were extracted from 16 sheep fecal samples (4 non-transgenic sheep, 6 VP1 transgenic sheep and 6 SB-SW transgenic sheep). And then PCR amplification with bacterial universal primers of V3 variable region of 16S rRNA were taken to get the fingerprint profile by denaturing gradient gel electrophoresis (DGGE) technology. The results showed that the DGGE profiles of the 16 fecal samples were highly polymorphic. The number of DGGE bands, considered indicative of the total species richness, did not vary predictably among the three different samples. The DNA sequences were analyzed and the dominant bacteria in sheep fecal were found to be Bacteroides, Clostridium, and Ruminococcus. Specially, non-transgenic sheep had more Alistipes finegoldii and Clostridium lentocellum, transgenic sheep with VP1 had more Clostridium drakei and Clostridium populeti and transgenic sheep with SB-SW had more Barnesiella intestinihominis and Clostridium ljungdahlii. So the PCR-DGGE technique can be applied to evaluate genetically modified (GM) animal potential risks to the environment.