Advanced Materials Research Vols. 884-885

Abstract: In this study, we succeed to establish the well in the well (WIW) culture system. Using this system we studied the effect of floating granulosa cells density on oocyte developmental competence. Results: Apoptosis rate of the floating cells were high (73.576.3%) and did not differ (P > 0.05) among follicles of different sizes. Hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WIW system (13.3%) and those cultured in the group system (16.3%). In 4-8mm follicles, oocyte developmental competence is higher (P < 0.05) in follicles with 1030×10⁵/mL floating cells than in follicles with < 10 or >30%×10⁵/mL floating cells.

Abstract: The roots of Raphanus sativus L., also called radish, has been used as a traditional anti-migraine drug in China for hundreds of years. However, its bioactive substances and pharmacological mechanism were still not very clear. In this study, two new natural products were isolated from the roots of Raphanus sativus L. by successive chromatographic procedures, such as open silica gel, Sephadex LH-20 and RP-18 column chromatograph. The structures of isolated compounds were elucidated as Cis-(1-methylazetidin-2-yl) methanol and cyclo- (4-methyl-Val-4-methyl-Val) on the basis of spectral data analysis, including MS and NMR (¹H-NMR, ¹³C-NMR, NOESY, HSQC and HMBC). The new natural products would be potential candidates for early anti-migraine drug study.

Abstract: Nerve conduits provide a promising strategy for peripheral nerve injury repair. Additional factors in nerve conduits such as cell adhesion molecules help to provide a more conducive microenvironment for nerve regeneration. In the present study, poly lactic acid (PDLLA) was modified by grafting Gly-Arg-Gly-Asp-Gly (RGD peptide) for fabricating new PDLLA/PRGD nerve conduits to promote nerve regeneration and functional recovery. In vivo, RGD addition improve the recovery efficiency of the transected nerve, that is, the sciatic function index (SFI) value of the PDLLA/PRGD group was significantly higher than those of group PDLLA. The regenerated nerves in PDLLA/PRGD group were more mature with significant elevations in both the cytoskeletal proteins expressions and the nerve growth factors expressions. Taken together, RGD peptide is one of the good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration.

Abstract: To study the dynamic variation of protein components and contents in Antheraea pernyi, 2D-PAGE, in this job, was used to investigate embryo development. Some special protein spots expression profiles changed obviously were analyzed by mass spectrometry. 2D-PAGE results showed that the protein spots increased from 370 at the development stage of 12 h to 422 at 300 h. Eighteen special protein spots were identified by LC-MS/MS, and 8 kinds of proteins are found to include heat shock proteins families, Vitellogenin, KK-42-binding protein, actin-depolymerizing factor 1, etc., which are mainly involved in some important processes. Analyze the function of those proteins, so as to accumulate basic information on embryonic development in A. pernyi.

Abstract: Objective: This study aimed to determine the composition and corrosion resistance of stainless steel and nickel titanium (NiTi) archwires and to compare Ni released from simulated standard fixed orthodontic appliances ligated with stainless steel and NiTi wires in artificial saliva at pH 5.14 and pH 6.69. in 4 weeks at 37oC. Materials and Method: Two commercial rectangular wires, 0.016 x 0.022 in size were studied, one Ormco stainless steel wire and another Ormco nickel titanium wire. Their composition was analyzed by an energy disporsive X-ray spectrometer (ESC) and their corrosion resistance was evaluated by a potentiodynamic polarization technique. For Ni release, the twenty-eight simulated fixed appliance sample sets were used, each corresponding to one half-maxillary arch. The samples were divided in 2 groups (14 sets per group). The first one was ligated to Ormco stainless steel archwires and the other one to Ormco NiTi archwires with elastomeric ligatures. Half sets of each group were immersed in 50 ml artificial saliva at pH 5.14 and the other half were immersed at pH 6.69. Ni release was quantified using flame atomic absorption spectrophotometry. Statistical analysis of variance (t-test) was determined on days 1, 4, 7, 9, 14, 21 and 28 comparing the amount of Ni released between groups. Results: Stainless steel wire was composed of Ni, Cr, Si, Mn and Fe while the nickel titanium wire was composed of Ti, Ni and Cr. NiTi wire has more percentage of Ni and therefore has less corrosion resistance than stainless steel wire. For Ni release, stainless steel and NiTi continuously increased at the time intervals for both pH levels. For group one, stainless steel wire had more Ni release at pH 5.14 and for the other group, NiTi wire had more Ni-release at pH 6.69. At 4 weeks, the Ni release of one half-maxillary arch was 1.383 ppm (1383 μg/l) at pH 5.14 and 1.079 ppm (1078 μg/l) at pH 6.69 for stainless steel wire while it was 1.221 ppm (1221 μg/l) at pH 5.14 and 1.267 ppm (1267μg/l) at pH 6.69 for NiTi wire. No significant difference was found in the amount of Ni release from stainless steel and NiTi wires at pH 5.14 and pH 6.69 in the different time intervals except on day 1 wherein the stainless steel archwire was significantly different (p<0.05) at pH 5.14 and pH 6.69. Conclusions: The NiTi wire had more Ni contain and less corrosion resistance than stainless steel wire. Stainless steel had more Ni release at pH 5.14 than pH 6.69 but NiTi had greater Ni release at pH 6.69 than pH 5.14.

Abstract: An ultrasensitive method for determination of L-dopa at picogram levels by flow-injection chemiluminescence (FI-CL) as presented for the first time, based on the quenching effect of levodopa (L-dopa) on the luminol-lysozyme reaction. It was found that the decrement of CL intensity was linearly proportional to the logarithm of L-dopa concentration ranging from 3.0 to 7.0 × 10³ pg mL^-1 (R = 0.9967), with the limit of detection (LOD) of 1.0 pg mL^-1 (3σ). The proposed procedure was successfully applied to the determination of L-dopa in pharmaceutical preparations, human saliva, serum, and urine samples with the recoveries ranging from 96.7% to 104.3% and RSDs less than 4.0% (n = 5).

Abstract: To provide the quality assurance for various samples test, fast testing methods of the performance of laser confocal micro-Raman spectroscopy system for cell analysis are developed. The silicon wafer was used on the performance test of LabRam INV system produced by Horiba JY. The silicon wafer can not only be used for system calibration (the band of 520.7cm^-1), but also for the evaluation of the system performance. The curves of the intensity at the band of 520.7 cm^-1 as a function of laser intensity radiated on the sample, pinhole and time were achieved. The results show that the intensity at the band of 520.7 cm^-1 change linearly with the laser intensity, which can be used to evaluate the laser intensity radiated on the sample. The intensity changes with pinhole can be used to determine the size of pinhole for different purpose. The stability of Raman system can be determined by the variation of the intensity at the band of 520.7 cm^-1 with time, which helps to decide whether it is suitable for other sample test.

Abstract: ADP-ribosylation factors (ARF) are ubiquitous regulators of vesicular membrane traffic in all eukaryotic cells. A full-length cDNA encoding an ARF was cloned from the cDNA library of Monascus purpureus. The cDNA was 1275 bp in length, contains a predicted 555 bp ORF that encodes 184 amino acids, the gene was designated MpARF. The deduced amino acid sequence showed high homology to ARF6 of Homo sapiens, ARFB of Aspergillus nidulans and ARF3p of Saccharomyces cerevisiae, including conserved N-terminal myristoylation site, GTP binding and GTP hydrolysis site, suggesting that the MpARF is a member of the ARF6 protein family. A typical G protein fold three-dimensional model of MpARF was built; the structure is similar to the structure of human ARF6. According to the functions of ARFB and ARF3p in fungi, we implicated that the MpARF would involved in hyphal polarized growth.

Abstract: Triazine Compound has many Potential Applications in Industries. in this Paper, a Convenient and Efficient Method for Preparation of 4,6-Trichloromethyl-2-(p-Acetobiphenyl)- 1,3,5-Triazine (compound 1) was Provided by Oxidition of 4,6-Trichloromethyl-2-(p-Ethylbiphenyl)- 1,3,5-Triazine (compound 4). Compound 4 was Prepared by Reacting 4-Cyano-4-Ethylbiphenyl with Trichloroacetonitrile.

Abstract: Muds and water samples collected from the Bohai Sea were selected to build seawater anaerobic ammonia oxidation reactor. The reaction volume was 18 L. The startup of reactor was divided into two stages. The first stage lasted five months and hydraulic retention time was 18L/7 d. The second stage lasted a month and hydraulic retention time was 18L/14 d. Ammonia and nitrite of influent and effluent were monitored. During the first stage, the removal rate of ammonia nitrogen was around 50% and the nitrite nitrogen increased by 50%. During the second stage, the removal rate of ammonia nitrogen reached more than 75% and the removal rate of nitrite nitrogen reached more than 90%. The removal ratio of ammonia nitrogen and nitrate nitrogen was about 1:1.17. These results suggested reactor start-up successful. Microbial community in reactor was monitored by polymerase chain reaction. Once the reactor started, specific bands of anaerobic ammonia oxidation microorganisms were found.