Adhesion and Proliferation of Chondrocytes on Nano- HA/PDLLA Scaffolds for Bone Tissue Engineering
In this paper, three different scale HA/PDLLA porous scaffolds, nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were successfully fabricated using solvent casting/particulate leaching method. Chondrocytes adhesion and proliferation on these scaffolds were investigated. In detail, the cells attachment rate and proliferation on nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were quantitatively evaluated by cytometry. The interaction between the scaffolds and chondrocytes were observed by optical microscope with HE staining and FE-SEM. The results exhibited that nano-HA/PDLLA scaffold has a modified cell adhesion property, and cells on the nano-scaffold grow much better both in biological and morphological characteristics than on the micro-HA/PDLLA and pure PDLLA scaffolds. This work suggested that nano-HA/PDLLA composite scaffold can significantly improved cell adhesion and proliferation tendency with the existing of nano-effects，and could be used as a potential scaffold material for bone defect repair.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
P.P. Chen et al., "Adhesion and Proliferation of Chondrocytes on Nano- HA/PDLLA Scaffolds for Bone Tissue Engineering", Key Engineering Materials, Vols. 309-311, pp. 919-922, 2006