Adhesion and Proliferation of Chondrocytes on Nano- HA/PDLLA Scaffolds for Bone Tissue Engineering

P.P. Chen; Sheng Min Zhang; L. Cheng; S.L. Huang; Jian Liu; W. Zhou; H. Gong; Q.M. Luo

doi:10.4028/www.scientific.net/KEM.309-311.919

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 309-311Adhesion and Proliferation of Chondrocytes on...

Adhesion and Proliferation of Chondrocytes on Nano- HA/PDLLA Scaffolds for Bone Tissue Engineering

Abstract:

In this paper, three different scale HA/PDLLA porous scaffolds, nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were successfully fabricated using solvent casting/particulate leaching method. Chondrocytes adhesion and proliferation on these scaffolds were investigated. In detail, the cells attachment rate and proliferation on nano-HA/PDLLA, micro-HA/PDLLA and pure PDLLA were quantitatively evaluated by cytometry. The interaction between the scaffolds and chondrocytes were observed by optical microscope with HE staining and FE-SEM. The results exhibited that nano-HA/PDLLA scaffold has a modified cell adhesion property, and cells on the nano-scaffold grow much better both in biological and morphological characteristics than on the micro-HA/PDLLA and pure PDLLA scaffolds. This work suggested that nano-HA/PDLLA composite scaffold can significantly improved cell adhesion and proliferation tendency with the existing of nano-effects，and could be used as a potential scaffold material for bone defect repair.

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Periodical:

Key Engineering Materials (Volumes 309-311)

Pages:

919-922

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.309-311.919

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Online since:

May 2006

Authors:

P.P. Chen, Sheng Min Zhang, L. Cheng, S.L. Huang, Jian Liu, W. Zhou, H. Gong, Q.M. Luo

Keywords:

Adhesion, Nanohydroxyapatite (HA), Osteocytes, Poly(D,L-Lactide) (PDLLA), Proliferation

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