In Vitro Evaluation of Hydroxyapatite-Containing Glass Coating on Zirconia
The aim of this study was to investigate the response of osteoblast-like cells (MC3T3-E1) to the surface of hydroxyapatite-containing glass coating on zirconia (HA-G-Zr) in comparison to yttria stabilized zirconia (Y-TZP). The MC3T3-E1 cells were cultured on HA-G-Zr and Y-TZP specimens in 24-well tissue culture plates. Surface properties were evaluated by X-ray diffractometry, Fourier tranfer infrared microscopy (FT-IR) and scanning electron microscopy (SEM). Cell proliferation in each well was measured by MTT assay. Cell morphology was observed by SEM. Cell differentiation was evaluated by alkaline phosphatase (ALP) activity and osteocalcin levels. After the glass coating on Y-TZP, X-ray diffraction peaks due to hydroxyapatite (HA) were observed clearly. HA-G-Zr appeared on surface of uneven and roughened state, wherein microcracks on the microns in width and many voids of several microns in size were present. Time-dependent proliferation and differentiation of MC3T3-E1 cells were found in all the specimens. MC3T3-E1 cells on HA-G-Zr plates showed higher differentiation than Y-TZP. These results demonstrated that HA-G-Zr showed better cellular biocompatibility than Y-TZP.
Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
D. Yamashita et al., "In Vitro Evaluation of Hydroxyapatite-Containing Glass Coating on Zirconia", Key Engineering Materials, Vols. 493-494, pp. 7-10, 2012