Enrichment Efficiency Optimization of Dielectrophoresis Cell Chip Based on Interdigitated Microelectrodes Array

Abstract:

At present, the majority dielectrophoresis chip designs optimize their performance by electric simulation and fluid field simulation. The lack of further cells force analysis and movement prediction results in a limited cell dielectrophoresis enrichment efficiency. The HepG2 hepatoma carcinoma cell movement under the action of dielectrophoresis force, fluid force and gravity was analyzed respectively by Comsol Multiphysics software. The preliminary optimization conditions for the cell enrichment were obtained. According to the simulation results, the experiments were carried out to investigate the enrichment effect of the HepG2 hepatoma carcinoma cells. The experiment result demonstrated the enrichment efficiency of HepG2 hepatoma carcinoma cells was reached to 88.89% under the 5 V amplitude and 4 MHz frequency sinusoidal signal excitation.

• Periodical: Key Engineering Materials (Volumes 645-646)
• Pages: 1255-1260
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.645-646.1255
• Online since: May 2015
• Authors: Wen Wen Gu*, Jie Huang
• Keywords: Cell Enrichment Efficiency, Dielectrophoresis Chip, Force Simulation, HepG2 Hepatoma Carcinoma Cells, Interdigitated Microelectrodes Array

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