Biocompatibility Evaluation of Cellulose Nanowhisker from Bamboo Fibers for Potential Applications of Drug Delivery In Vitro

Abstract:

The biocompatibility of the cellulose nanowhisker (CNW) from cellulase hydrolysis was evaluated in vitro for potential applications in drug delivery system. The assessments were comprised of cytotoxicity analysis and proliferation of human osteosarcoma MG-63 cells that were cultured with the resultant CNW. Some morphological and physico-chemical characteristics of the CNW were investigated. The cellulase hydrolysis conditions were optimized at a cellulase dosage of 0.01 mL/g dried fibers, a hydrolysis temperature of 60 °C, hydrolysis time of 3 h and bamboo fiber concentration of 2 wt%. Under these conditions, the as-prepared CNW retained more properties similar to the original bamboo fibers than those fabricated by sulfuric acid hydrolysis. Indirect cytotoxicity test of the CNW revealed it non-toxicity to the cells. The proliferation of the MG-63 cells with the CNW from cellulase hydrolysis was better than that from sulfuric acid hydrolysis. These results together with the fact that the cellulose hydrolysis preparation of CNW is simple and inexpensive make it a good candidate for the design of oral drug delivery device.

• Periodical: Materials Science Forum (Volume 787)
• Pages: 468-473
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.787.468
• Online since: April 2014
• Authors: Yong Zhang*, Lan Ma, Ye Li Yu, Ju Ming Yao
• Keywords: Bamboo, Biocompatibility, Cellulose, Delivery, Drug, Fiber, Nanowhisker

References

[1] Heath L., Thielemans W., Cellulose nanowhisker aerogels, Green Chem. 12 (2010) 1448-1453.

DOI: 10.1039/c0gc00035c

[2] Moon R.J., Martini A., Nairn J., Simonsen J., Youngblood J., Cellulose nanomaterials review: structure, properties and nanocomposites, Chem. Soc. Rev. 40 (2011) 3941-3994.

DOI: 10.1039/c0cs00108b

[3] Lahiji R.R., Xu X., Reifenberger R., Raman A., Rudie A., Moon R.J., Atomic force microscopy characterization of cellulose nanocrystals, Langmuir 26 (2010) 4480-4488.

DOI: 10.1021/la903111j

[4] Zhu J.Y., Sabo R., Luo X.L., Integrated production of nano-fibrillated cellulose and cellulosic biofuel (ethanol) by enzymatic fractionation of wood fibers, Green Chem. 13 (2011) 1339-1344.

DOI: 10.1039/c1gc15103g

[5] Park, H.P., Oh, K.W., Kim, S.H., Reinforcement effect of cellulose nanowhisker on bio-based polyurethane, Compos. Sci. Technol. 86 (2013) 82-88.

DOI: 10.1016/j.compscitech.2013.07.006

[6] Male K.B., Leung A.C., Montes J., Kamen A., Luong J.H., Probing inhibitory effects of nanocrystalline cellulose: Inhibition versus surface charge, Nanoscale 4 (2012) 1373-1379.

DOI: 10.1039/c2nr11886f

[7] Kou X., Yang R., Zhao J., Lu J., Liu Y., Enzymatic saccharification and L-lactic acid fermentation of corn stover pretreated with liquid hot water by rhizopus oryzae, BioResources 8 (2013) 4899-4911.

DOI: 10.15376/biores.8.4.4899-4911

[8] Lam E., Male K.B., Chong J.H., Leung A.C., Luong J.H., Applications of functionalized and nanoparticle-modified nanocrystalline cellulose, Trends Biotechnol. 30 (2012), 283-290.

DOI: 10.1016/j.tibtech.2012.02.001

[9] Zhang Y., Lu X.B., Chen W., Lv W.J., Preparation and characterization of nanocrystalline cellulose from bamboo fibers by controlled cellulase hydrolysis, Proceedings of 4th ICPPB 1 (2012), 228-232.

[10] Updegraff D.M., Semi microdetermination of cellulose in biological materials, Anal. Biochem. 32 (1969), 420-429.

[11] Neamnark A., Sanchavanakit N., Pavasant P., Bunaprasert T., Supaphol P., Rujiravanit R., In vitro biocompatibility evaluations of hexanoyl chitosan film, Carbohyd. Polym. 68 (2007) 166-172.

DOI: 10.1016/j.carbpol.2006.07.024