Characterization of Porous Hyaluronan/β-TCP Scaffolds Prepared through Heterogeneous Crosslinking

Abstract:

Porous Hyaluronan/β-tricalcium phosphate composite scaffolds were synthesized through lyophilizing and subsequent heterogeneous crosslinking method. The morphology of the composite scaffolds were investigated by scanning electron microscopy (SEM). The swelling behavior, mechanical property, degradation behavior and cell adhesion ability of samples were also studied. The results revealed that hyaluronan mainly contributed to the polymer matrix and water adsorption, whereas β-TCP acted as a reinforcement to strengthen the porous structure, while too much β-TCP would make the structure collapse. The pose size of obtained scaffolds ranges from100μm to 200μm and the porosity decreased with the increase of β-TCP content. The degradation behavior and cell adhesion test indicated that increasing hyaluronan concentration can effectively improve the degradability of scaffolds and the incorporation of β-TCP improved the cell adhesion performance. Thus a simple way to prepare hyaluronan-based composite scaffolds was provided, which could be potentially used as an tissue engineering material.

• Periodical: Materials Science Forum (Volume 914)
• Pages: 29-36
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.914.29
• Online since: February 2018
• Authors: Rui Min Zhao*, Xian Ya Xue, You Fa Wang
• Keywords: Composite, Heterogeneous, Hyaluronan, Porous Scaffold, β-TCP

References

[1] D.W. Hutmacher: Biomaterials, Vol. 21 (2000) No. 24, p.2529.

[2] W. Ji, Y. Sun, F. Yang, et al.: Pharmaceutical Research, Vol. 28 (2011) No. 6, p.1259.

[3] S. Saravanan, R. S. Leena, N. Selvamurugan: International Journal of Biological Macromolecules, Vol. 93 (2016) Part. B, p.1354.

[4] B. Balakrishnan, R. Banerjee: Chemical Reviews, Vol. 111 (2011) No. 8, p.4453.

[5] L. Lapčík Jr., L. Lapčík, S.D. Smedt, et al.: Chemical reviews, Vol. 98 (1998) No. 8, p.2663.

[6] L.A. Solchaga, J.U. Yoo, M. Lundberg, et al.: Journal of Orthopaedic Research, Vol. 18 (2000) No. 5, p.773.

[7] A. K. Jha, R. A. Hule, T. Jiao, et al.: Macromolecules, 2009, 42(2): 537-546. Vol. 42 (2009) No. 2, p.537.

[8] C. Guarise, M. Pavan, L. Pirrone, et al.: Carbohydrate Polymers, Vol. 88 (2012) No. 2, p.428.

[9] E. Karousou, S. Misra, S. Ghatak, et al.: Matrix Biology, Vol. 59 (2017) , p.3.

[10] J.J. Young, K.M. Cheng, T.L. Tsou, et al.: Journal of Biomaterials Science, Polymer Edition, Vol. 15 (2004) No. 6, p.767.

[11] M.R. Nejadnik, X. Yang, M. Bongio, et al.: Biomaterials, Vol. 35 (2014) No. 25, p.6918.

[12] V. Mourino, J. P. Cattalini, J. A. Roether, et al.: Expert Opinion on Drug Delivery, Vol. 10 (2013) No. 10, p.1353.

[13] C.E. Schanté, G. Zuber, C. Herlin, et al.: Carbohydrate Polymers, Vol. 85 (2011) No. 3, p.469.

[14] J. Y. Lai, D. H. Ma, H. Y. Cheng, et al.: Journal of Biomaterials Science, Vol. 21 (2010) No. 3, p.359.

[15] I. R. Serra, R. Fradique, M.C.S. Vallejo, et al.: Materials Science and Engineering: C, Vol. 55 (2015), p.592.

[16] Y. Takahashi, M. Yamamoto, Y. Tabata: Biomaterials, Vol. 26 (2005) No. 17, p.3587.

[17] H. Cao, N. Kuboyama: Bone, Vol. 46 (2010) No. 2, p.386.

[18] K. Yang, J. Zhang, X. Ma, et al.: Materials Science and Engineering: C, Vol. 56 (2015) , p.37.

[19] Y. Zhou, L. Xu, X. M Zhang, et al.: Materials Science and Engineering: C, Vol. 32 (2012) No. 4, p.994.

[20] F. Zhang, C. He, L. Cao, et al.: International Journal of Biological Macromolecules, Vol. 48 (2011) No. 3, p.474.

[21] A. Sionkowska, J. Kozlowska: International Journal of Biological Macromolecules, Vol. 52 (2013), p.250.

[22] A. La Gatta, C. Schiraldi, A. Papa, et al.: Carbohydrate Polymers, Vol. 96 (2013) No. 2, p.536.