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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1120-1121Production of Cellulase by Aspergillus niger FC-1...

Production of Cellulase by Aspergillus niger FC-1 and its Application in Hydrolysis of Corncob Residues

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Abstract:

In this study,the cellulase-producing ability of Aspergillus niger FC-1 through solid-state fermentation (SSF) and characteristics of the cellulase were investigated. The maximum activities of total cellulase (FPase) and endoglucanase (CMCase) were 8.2 and 31.5 IU per gram of dried substrate respectively after 96-h incubation period. The activities of both FPase and CMCase produced by Aspergillus niger FC-1 exhibited the optimal values at pH 5.5 and 50°C(as shown in Fig.2). Thermostability and pH stability of the enzymes were respectively appreciable at temperature ranging from 45°C to 55°C, pH ranging from 5.0 to 5.5. In addition, with an optimal 1:10 (w/v) substrate to moisture ratio (a cellulase loading of 8.5 FPU per cellulose), the glucose concentration was as high as 36.6 g glucose l^-1 for a 48 h hydrolysis of corncob residues.

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Periodical:

Advanced Materials Research (Volumes 1120-1121)

Pages:

891-896

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1120-1121.891

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Online since:

July 2015

Authors:

Hui Qin Shi, Zhe Wei Zhao, Wei Yang, Di Wu, Yi Zhao, Jin Zhao, Xiao Min Fang, Lu Lu Xie, Xiang Yu Tian, Jian Wu, Gui Fu Dai*

Keywords:

Aspergillus niger FC-1, Cellulase, Corncob Residues, Enzymatic Hydrolysis

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© 2015 Trans Tech Publications Ltd. All Rights Reserved

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* - Corresponding Author

[1] J. Goldemberg, Ethanol for a sustainable energy future, Science. 315 (2007) 673-676.

[2] R.C. Kuhad, A. Singh, K.E. Ericksson, Microorganisms and enzymes involved in the degradation of plant fiber cell walls, Adv Biochem Eng Biotechnol. 57 (1997) 45–125.

[3] N. Bansal, R. Tewari, R. Soni, S.K. Soni, Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues, Waste. Manag,. 32 (2011) 1341–1346.

DOI: 10.1016/j.wasman.2012.03.006

[4] Y.B. Qu, Progress in basic and technological research of enzyme system for lignocellulosics biodegradation, Journal of Shandong University (Natural Science). 46 (2011) 160-170.

[5] C. Lei, J. Zhang, L. Xiao, J. Bao, An alternative feedstock of corn meal for industrial fuel ethanol production: delignified corncob residue, Bioresour Technol. 167 (2014) 555–559.

DOI: 10.1016/j.biortech.2014.06.005

[6] R. Sun, X. L . Song, R.C. Sun, J.X. Jiang, Effect of lignin content on enzymatic hydrolysis of furfural residues. Bioresources. 6 (2011) 317–328.

DOI: 10.15376/biores.6.1.317-328

[7] E. Schuster, N. Dunn-Coleman, J.C. Frisvad, P.W. Van Dijck, On the safety of Aspergillus niger – a review, Appl Microbiol Biotechnol. 59 (2002) 426–435.

[8] S.Y. Jiang, H.Q. Shi, M.F. GAO, Y.P. Liu, X.M. Fang, G.F. Dai, G.Y. Zhu, X.Y. Deng, Y.F. Zhang, B. Zhang, X.D. Shang, J. Wu, Cellulase Production by Submerged Fermentation Using Biological Materials of Corncob residue with Aspergillus niger FC-1, Advanced Materials Research, 648 (2013).

DOI: 10.4028/www.scientific.net/amr.648.116

[9] U.F. Rodríguez-Zúñiga, V.B. Neto, S. Couri, S. Crestana, C.S. Farinas, Use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation, Applied Biochemistry and Biotechnology. 172 (2014).

DOI: 10.1007/s12010-013-0678-0

[10] T.K. Ghose, Measurement of cellulase activity, Pure and Applied Chemistry. 59 (1987) 257-268.

[11] M.S. Chandra, B. Viswanath, B.R. Reddy, Cellulolytic enzymes on lignocellulosic substrates in solid state fermentation by Aspergillus niger, Indian J. Microbiol. 47 (2007) 23–3282.

DOI: 10.1007/s12088-007-0059-x

[12] R.R. Singhania, R.K. Sukumaran, A.K. Patel, C. Larroche, A. Pandey, Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases, Enzyme and Microbial Technology. 46 (2010).

DOI: 10.1016/j.enzmictec.2010.03.010

[13] R.K. Sukumaran, R.R. Singhania, G.M. Mathew, A. Pandey, Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bioethanol production, Renew Energ. 34 (2009) 421–424.

DOI: 10.1016/j.renene.2008.05.008

[14] Y. Feng, J. Jiang, L. Zhu, L. Yue, J. Zhang, S. Han, Effects of tea saponin on glucan conversion and bonding behaviour of cellulolytic enzymes during enzymatic hydrolysis of corncob residue with high lignin content, Applied biochemistry and biotechnology. 172 (2014).

DOI: 10.1186/1754-6834-6-161

[15] L.X. Bu, Y. Xing, H.L. Yu, Y.X. Gao, J.X. Jiang, Comparative study of sulfite pretreatments for robust enzymatic saccharification of corncob residue, Biotechnology for Biofuels. 5 (2012) 87.

DOI: 10.1186/1754-6834-5-87