The spinel nano-Li4Ti5O12 was prepared by hydrothermal reaction method. The crystal structure, morphology and charge/discharge capacities were characterized by powder X-ray diffraction and Scanning electron microscopy and program-controlled tester respectively. It showed that lithium titanate synthesized by the direct hydrothermal method has a uniform particle size distribution of about 100nm in cubic shape. Investigated the influence of different carbon sources on the structure and electrochemical properties. It was found that the discharge capacities could reach 172.2, 159.8, 156.2 and 148.3mAh/g at 0.2C, 1C, 2C and 5C rates, respectively.