Advanced Materials Research Vols. 550-553

Abstract: Capsaicin microencapsules were prepared by complex coacervation with gelatin and carboxymethyl cellulose (CMC). The process conditions were optimized by Orthogonal design on the base of single factor test. As wall material concentration (WMC), gelatin/CMC rate(GCR), core/wall rate(CWR) and pH, are 1%, 9:1, 1:1 and 4.5-4.6 respectively, the encapsulation yield is 92.53% and efficiency is 91.23%. The analysis of capsaicin microcapsule showed that water content is 3.12％ and the repose angle is 43.8°. The microcapsule particle and microstructure were valued by scanning electron microscope (SEM). FT-IR spectrum proved that the coacervate is formed successfully.

Abstract: Objective: To study the association between neuronal injury following status epilepsy and expression of P-glycoprotein, discuss the association between intractable epilepsy and neuronal injury. Method: To establish a mice status epilepticus model by lithium-pilocarpine injected intraperitoneal, The mice were randomly divided into control group 6h, 12h, 1d, 3d, 5d and 7d after status epilepticus(SE) .To research the neuronal injury by image analysis method, expression of P-gp in blood was studied by flow cyometry .To analyze the association between neuronal injury and expression of P-gp by Pearson statistics. Result: The neuronal injury was observed after status epilepsy. Neuronal injury is most obvious 3-7 days after status epilepsy, expression of P-gp increased simultaneously. The severity of neuronal injury has a positive correlation to expression of P-gp. Conclusion: Association between intractable epilepsy and neuronal injury exists.

Abstract: To prepare ultra-fine particles and offer correlative applications such as the micronization of drugs with rapid expansion of supercritical solution (RESS), the first work in measuring the solubility of risocaine has been carried out at different operation conditions in this paper. The trend of solubility due to changes in specific operational parameters has been examined. The results of pressure (9-30 MPa) and temperature (308-328K) effecting on solubility show that the solubility increases mainly along with the increasing density. With applying different models to correlate the solubility data, we found Chrastil models had better correlation effects than the Peng Robinson EOS model, Mendez-Santiago and Teja equation model, with providing a nearly perfect average absolute relative deviation (AARD) of 0.0596. In the second part of work, RESS was applied to prepare risocaine particles at five different process conditions, including extraction temperature (308-328K), extraction pressure (9-30MPa), nozzle temperature (100-120°C), nozzle diameter (0.1-0.4nm) and spray distance (2-4cm).The size and morphology were determined by scanning electron microscopy (SEM). On the basis of the different experimental operation conditions, granular, filmily and threadlike particles with diameter (1-100nm) were obtained, it was also demonstrated that a successful size reduction of risocaine particles.

Abstract: Carbon microspheres (CMSs) were used as the carrier to prepare the Ag-loading CMSs (Ag/CMSs) antibacterial agent through the method of chemical adsorption. The morphologies and structures of modified CMSs were characterized by using the field emission Scanning Electron Microscope (SEM). The results showed that silver was absorbed on the surface of CMSs. The bacterial inhibition ring experiment showed that Ag/CMSs had good antibacterial activity against Staphylococcus aureus and Escherichia coli, meanwhile the diameters of the bacterial inhibition rings were 19 mm against Staphylococcus aureus and 21 mm against Escherichia coli, respectively.

Abstract: Purpose To develop a novel complex antimicrobial agent and determine the optimal components of the composite antimicrobial agents and its antimicrobial activity in vitro. Methods According to antimicrobial mechanisms，antibacterial spectrums，physical and chemical properties and applicabilities of existing antimicrobial agents in clinical use, select out cefoperazone sodium, sulbactam sodium and cephradine as the basic components to make a novel complex antimicrobial agent. Utilize yeast, staphylococcus aureus and E. coli bacteria as test bacteria. Do the three factors four-level orthogonal experiments by the maximum amount, the middle amount, low amount and Minimum amount of the three-component agent to research the optimum ratio of the drug. Measure the titer of the compound antimicrobial agent by the way of tube-plate method (2 doses). With known contents of Penicillin Sodium for Injection as control, and determine its minimum inhibitory concentration against staphylococcus aureus, E. coli and yeast by using the agar doubling dilution method. The experimental results were analysized by statistical analysis software SPSS16.0. Results The results of the three factors four-level orthogonal experiments indicate the optimum ratios of Cefoperazone Sodium, Sulbactam Sodium and Cephradine against E. coli, yeast and staphylococcus aureus were 2:2:3, 1:2:2 and 2:6:5, their titers were 1353.9U/mg, 982.7U/mg and 1015.5U/mg. With the highest titer proportion 2:2:3 as the composition of the antimicrobial compound. This compound antimicrobial agent had a good antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and Fungi, its minimal inhibitory concentration (MIC) against staphylococcus aureus, E. coli and yeast were 2.000μg/ml, 0.500μg/ml and 16.000μg/ml. Conclusion This research acquires a composite of antibiotics. This antimicrobial compound has a broader spectrum and higher antimicrobial activity in vitro comparing with traditional common single antibiotics, and it especially has a good antimicrobial activity against fungi. The results set a scientific foundation for enriching clinical medicines.

Abstract: In this paper, triphosgene respectively reacting with o-nitrophenyl aniline, m-nitrophenyl aniline and p-nitrophenyl aniline to synthesize corresponding isocyanates. The effects of reactants molar ratio, reaction time and reaction temperature on the yield were investigated. As a result, using 1,2-dichloroethane as reaction solvent, the optimum molar ratio of the three kinds of nitroaniline and triphosgene were all 2.5:1, the optimum reaction time of synthesis of o-nitrophenyl isocyanate, m-nitrophenyl isocyanate and p-nitrophenyl isocyanate respectively was 6h, 5.5h and 5h; the optimum reaction temperature of synthesis of the three kinds of nitrophenyl isocyanates were all 75°C. Under the optimal conditions, the yield of o-nitrophenyl isocyanate, m-nitrophenyl isocyanate and p-nitrophenyl isocyanate could respectively be 80.3%, 83.7% and 83.5%. The products were characterized by meltingtest, ebulliometry, IR, and HPLC

Abstract: Taurine (Tau) was widely used in adjusting body normal physiological function and enhancing the ability of resisting all kinds of stress in clinical medicine and sports nutrition. This article collected the literatures of recent 20 years about the application of taurine in animal and human being. According to the chemical structure, amino acids in taurine’s molecular structure played a main role in regulating physiological function. Amino was the key and even the only functional group in biochemical reaction. Schiff’s Reaction between amino and carbonyl in reactive carbonyl compounds was that taurine played a variety biological function of molecular basis in cells.

Abstract: In this work, exopolysaccharide (EPS) production by the yeast Rhodotorula glutinis was investigated. The results suggest that the synthesis of EPS in batch fermentations was enhanced by using a low C/N ratio to stimulate fast cell growth during the exponential phase and by adding glucose to stationary phase cultures to provide excess carbon for EPS formation. The apparent viscosity and molecular weight of EPS were found to decrease with increasing fermentation time due to the partial hydrolysis of EPS by the acidic fermentation broth (pH 1.8). Maintaining stationary phase cultures at pH 4 was found to be effective in minimizing acid hydrolysis and producing EPS with high molecular weight and apparent viscosity.

Abstract: Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.

Abstract: In this paper the survival rate of Saccharomyces cerevisiae cells after the formic acid treatment of different concentration was determined firstly, and cells of Saccharomyces cerevisiae were labeled with fluorescent probes of reactive oxygen species (ROS) and treated with formic acid, then intracellular reactive oxygen species was detected with confocal microscopy and flow cytometer. The results show that formic acid can lead to the rapid burst of intracellular reactive oxygen species. We speculated that the outbreak of the formic acid-induced reactive oxygen species and corresponding oxidative damage is the leading cause of the yeast cells death.