Advanced Materials Research Vols. 581-582

Abstract: The lead-zinc mine is a low grade of refractory oxide mineral. The grade of lead is 2.84%, with the oxidation rate of 90.95%.The grade of zinc is 3.223%, and with the oxidation rate of 87.9%.The grade of silver is 99.5g/t.This test used the silver-lead sulphide flotation at first, then depressing zinc oxide and floating lead oxide mineral. As a result, in silver-lead sulphide concentrate, the silver grade for 1180 g/t, lead 11.45%. Lead and silver recovery rate were 18.41% and 51.14% respectively. Lead oxide concentrate grade is 32.59%, and the silver is 518g/t. Lead and silver recovery rate were 68.34% and 29.18%. Zinc oxide concentrate grade is 32.65%, including 1.14% lead; zinc recovery rate is 80.11%.

Abstract: A detailed investigation of the effects of Nd on the microstructure, mechanical properties and electrochemical properties of lead anode in 160g.L^-1 at 35°C was carried out. Galvanostatic polarization and Chronopotentiometry (CP) were used to study the electrochemical behavior ( such as anodic potential, corrosion rate and the composition of passive film) of the Pb and Pb-Nd anodes. The metallographic structure and passive film morphology of Pb and Pb-Nd anodes were observed and analyzed using polarizing microscope and scanning electronic microscopy (SEM), respectively. The experimental results show the grains become smaller and aggregation of PbxNdy become severe as the content of Nd increases. The addition of Nd enhances the formation of PbO₂, inhibits the formation of PbSO₄ and PbO and reduces the anodic potential. However, Holes presenting on rough passive film of high Nd content Pb-Nd anodes accelerate the corrosion.

Abstract: A new technology for preparing titanium-rich material from modified titanium material by microwave selective leaching is studied in this paper. The systemic conditional experiments are conducted on the flow sheet and the optimum technological parameters of microwave leaching including leaching temperature of 160°C, concentration of hydrochloric acid 10%, ratio of liquid to solid 10:1, leaching time of 120min. The content of TiO2 of titanium-rich material is 96.08% under the optimum condition.

Abstract: The high ash coke breeze was leaching by two-stage chemical leaching. X-ray diffraction (XRD) and X-Ray Fluorescence (XRF) were employed to characterize the mineral phase and the element content respectably. More than 80% ash has been removed from the coke breeze by two-stage leaching. The removal of Si and Al was significantly affected by the temperature of alkali leaching. The mineral phase of the result coke breeze is similar to that of the raw ash except with cristobalite and sodium mica.

Abstract: The removal efficiency of degrading flora which has been constructed is investigated in biological carbon process through the variation of COD and chromaticity. The results show that the efficient degrading strains can reproduce on the activated carbon quickly；the biological activated carbon reactor，in which degrading flora to coking waste water are inoculated，is adopted to carry out the treatment of wastewater，in such a way the COD and chromaticity in outflow water can remain at a lower level (with COD about 50mg/L and chromaticity about 50 degree). The average COD removal rate is 71% and the chromaticity removal ratio is 95%.

Abstract: A spatial quantitative combination seek-different model of hydrology geochemistry elements was applied for the quantitative analysis of underground water elements in Shuangliao endemic fluorosis area, Jilin province, China. According to main factors analysis, chemical types plots of underground water, and distributions of endemic fluorosis area, the results indicated there was an obvious positive correlation between F- and NO2-, F- and K+, which showed no endemic fluorosis in this area; while there was a weak negative correlation between Cl-, NO3-, Ca2+, Mg2+, and F-. HCO3- and Na+ performed a strong positive correlation between HCO3- and Na+, but showed a negative correlation with F-, which suggested a low incidence in this area. This result was probably due to a lot of Ca2+, Mg2+ cation in water, which had an antagonism effect on F-, and the concentration of F- was relative low. The effect of endemic fluorosis under chemical conditions of underground water was contributed to the comprehensive effect of antagonism and synergism.

Abstract: Mussel adhesive protein has significant potential application in the field of medical adhesion. Genetic engineering method is gaining more and more attention, by which mussel adhesive protein can be heterologously expressed. In order to improve the expression efficiency of mussel adhesive protein with heterologous recombinant, it is reported that the compatible solutes Ectoine promoted to expression of adhesive protein on Pichia pastoris GS115. In this study, the adhesive protein gene msfp-1 from Mytilus sp. JHX-2002 was transformed into P. pastoris GS115. Inducement expression of adhesive protein Msfp-1 with various methanol concentrations was investigated. The promotion of Ectoine on the expression level of recombinant protein was studied. The results showed that adhesive protein Msfp-1 was induced with methanol on the recombinant GS115/msfp-1. The optimal concentration of methanol was 1% on heterologous expression. In the inducement expression phase of Msfp-1 with methanol, Ectoine could play a promotion role on expression of heterologous proteins. When the concentration of methanol was 1.5% and the addition of Ectoine was 1.5 mM, the expression of Msfp-1 was up to 2.1 g/L. Compared to fermentation broth without Ectoine, the expression was increased by 61.5%. Ectoine has an important promotion in the efficient expression of mussel adhesive protein on P. pastoris GS115.

Abstract: Disintegrants had influence on disintegration time and dissolution for fast disintegrating tablet (FDTs). Therefore, the purpose of this study was to evaluate the effect of disintegrant type on the characteristics of FDTs. In this research, the tablets were fabricated by direct compression with the compression force of 1.5 tons and using the different disintegrants components (chitin, chitosan, xylitol, microcrystalline celluloses, white bentonite and magnabite F). The physical properties of these tablets were determined. The xylitol tablets were rapidly disintegrated within 7+1 s, whereas that of the others was longer than 30 mins. The tablets containing the mixture of xylitol and other materials were subsequently fabricated since the hardness of the xylitol tablet was very poor. The disintegration time and wetting time of tablets containing 9:1 xylitol:Avicel PH101 was shorter than that of the others. The contact angle of tablets containing xylitol and Avicel PH101 at the ratio of 9:1 was lowest and surface free energy (SFE) of them was highest compared with the other formula. Moreover, scanning electron microscope (SEM) displayed that xylitol and tablets containing 9:1 xylitol:Avicel PH101 had no disordered arrangement, therefore it could promote the disintegrating property effectiently.

Abstract: Purpose: To search for the change of fibrinolytic activity of different forms of nattokinase when they were treated in human digestive juice, artifical gastric and intestinal juice differently, and to lay the foundation of a proper pharmaceutical dosage form of nattokinase. To provide direct evidences that Nattokinase products can be administered orally and to screen a suitable dosage form for oral administration of nattokinase.Methods: The non-crushed sample was prepared through the solid fermentation of soybean meal at 37°C for 36 h, and evaporation at 70°C for 8 h, The fine and ultra-fine powder were gotten from the non-crushed sample by the micronizer and ultra micronizer. Nattokinase extract was the supernatant liquid of 0.9% saline of non-crush samples. The nattokinase’s fibrinolytic activity of non-crushed sample, fine powder, ultra-fine powder and extract treated in artifical and human digestive were measured by the Fibrin plate method. Results: Three kind of solid form samples still remain about 80% fibrinolytic activity in artificial gastric juice for 4 h, and the fibrinolytic activity of nattokinase extract is 48.7% at the end of treatment. Treated in artificial gastric juice for 4 hours, then handled in artificial intestinal fluid for 4 h, the final fibrinolytic activity of fine and ultra fine powder is 47.0% and 51.0%. The fibrinolytic activity of extract is 79.8% at the end of treatment in human digestive juice for 4 h. At the beginning of treatment in human digestive juice the enzyme activity of fine and ultra fine powder is 130.8U/mL and 132.1U/mL, however, after treatment for 4 h the enzyme activity is 158.7U/mL and159.6U/mL, which is near to the total enzyme activity of sample. Conclusion: Nattokinase products can be administered orally, and solid preparations of nattokinase are more suitable for oral route. The optimum dosage form for oral administration of nattokinase is enteric-coated capsules containing ultra fine powder.

Abstract: In order to investigate the ability of Pteris vittata restoring Zn polluted soil, water cultured experiment is utilized to study the enriching ability of Pteris vittata to Zn, and the detoxification mechanism of Pteris vittata to Zn. The experimental results show that Pteris vittata has certain enriching ability to Zn,and the translation coefficient of Zn in Pteris vittata reaches 1.0. With the increasing of Zn application amount, chlorophyll and soluble sugar contents of Pteris vittata show ascending first and then descending,O2-• and MDA contents continuously ascend,starch content continuously descends.These results show that soluble sugar, starch,O2-• and MDA have detoxification effect,and can enhance the resistance of plant to heavy metal.