Advanced Materials Research Vols. 641-642

Abstract: The effects of different lighting energy and different radiant power on the properties of delayed luminescence of beans were discussed. It is showed when lighting energy is from 2 to 25mJ, initial intensity A changes very much from 1200 to 8600. When lighting energy is from 200 to 600 mJ, initial intensity A has little change. Initial intensity A is in a linear relation with radiant power.

Abstract: In order to study the toxicity mechanism of plant VOCs of four kinds of terpene monomers(α-terpineol,α-pinene,β-pinene,1,8-cineole), the activities of acetylcholinesterase in Zophobas morio larvae were treaded with spectrophotometer at different time (12h, 24h, 36h, 48h). The results showed that four kinds of terpene monomers (α-terpineol, β-pinene, α-pinene, 1,8-cineole) had high biological activity in Zophobas morio, which can inhibit the activity of acetylcholinesterase(AchE) effectively．

Abstract: The direct anti-tumor T-7 peptide of tumstatin was obtained to study its effect on human hepatoma cells (HepG-2) and human umbilical vein endothelial cells (ECV304). T-7 peptide was synthesized and its purity was up to 98.5%. MTT assay, growth curve, transmission electron microscopy(TEM) were used to detect the proliferation inhibition and pro-apoptotic function. MTT experiments and growth curve experiments showed that the survival of human hepatoma cells (HepG-2) decreased in a time- and dose-dependent way with the concentration of T-7 peptide increased. T-7 peptide can induce apoptosis of HepG-2 significantly. Apoptosis features were observed by TEM. However, the inhibition of T-7 peptide on human umbilical vein endothelial cells (ECV304) was weaker. Experiments showed thatT-7 peptide can affect HepG-2 cells. It had a certain therapeutic effect on human hepatocellular carcinoma. T-7 peptide have little effect on ECV304 cells, which means it didn’t influence the formation of tumor angiogenesis and normal cells

Abstract: Objective: In order to study the effect of different intensity exercise on serum nitric oxide and nitric oxide synthase of elderly men, and look for the most appropriate exercise intensity for them which can prevent cardiovascular disease. Methods: 36 men who are 55 to 62 years old, were randomly divided into the low intensity group(L group,n=12), the moderate-intensity group(M group,n=12) and the high-intensity group(H group,n=12). During the period for testing, they walked for 12 weeks, 4 times per week, 45 minutes for each time,target heart rate is 100 ~110 beats/min(L group), 110 ~120 beats/min(M group), 120~ 130 beats/min(H group). With an over-night fast, all the subject venous blood samples were drawn by 4ml before and after the exercise. The blood samples were centrifuged and separated immediately, then serum nitric oxide content, TNOS activity, cNOS activity, iNOS activity were measured.Results:In low-intensity group, serum NO content, TNOS activity, cNOS activity were significantly increased than pre-exercise (P<0.05); in moderate-intensity group,serum NO content,TNOS activity,cNOS activity were very significantly increased than pre-exercise (P <0.01);the indicators of high-intensity exercise group did not change significantly. Conclusion: we recommend that a more appropriate exercise intensity is 100-120 beats /min for elderly during fitness exercise, and especially 110-120 beats/min. Such intensity exercise can increase NO content in vivo, thus effectively prevent the occurrence of cardiovascular disease.

Abstract: Rare ginsenoside C-K was prepared using enzymatic transformation with snailase from main ginsenoside Rb1 at the following conditions: material ratio 62.14:10, pH 4.0, incubation at 46.40 °C for 15 h. After chromatography by silica gel column and crystal formation, C-K with more than 95% purity was obtained from the production of enzymatic transformation. The yield of C-K reached 63.02% after optimization, representing an about 9% increase than before. In addition, C-K we prepared exhibited an obvious inhibitory effect on the proliferation of human lung adenocarcinoma cancer cell A549 and human hepatoma cell HepG-2.

Abstract: In the present study, pyrolysis characteristics of corn straw pellet were studied in biomass pyrolysis test bench. The influence of pellet diameter on pyrolysis products was investigated. The results showed that: with the increase of pellet diameter, the yield of tar decreased ,and the yield of char and gas increased, and char density increased too. The results has significant theoretical guidance on the biomass gasification and preparation of char using biomass pellets.

Abstract: During the initial steps of porphyrins biosynthesis, two molecules of 5-aminolevulinic acid are asymmetrically condensed to porphobilinogen by aminolevulinate dehydratase (ALAD). To investigate the relationship between the mycelium development and gene expression for ALAD in Monascus purpureus, the cDNA coding for ALAD of M.purpureus was isolated. The recombinant ALAD protein was expressed in E. coli BL21 (DE3) cells and subsequently purified using affinity chromatography. We analyzed the phylogenetic variation in metal ion usage by ALAD enzymes, and suggested that MpALAD utilize zinc to act in catalytic site. Transcriptional expression of MpALAD during M.purpureus liquid cultivation process was assayed by RT-PCR analysis on mycelium at two distinct development stages. With the transition of mycelium color from white to red, a decrease in the transcript level for MpALAD gene was observed. The results revealed that the transcriptional expression of MpALAD is developmentally regulated in M.purpureus.

Abstract: The linoleic acid isomerase enzyme from Propionibacterium acnes, which catalyzed the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating trans-10, cis-12 conjugated linoleic acid (t10, c12 CLA) was cloned and overexpressed in Escherichia coli using expression system of pCold-SUMO. The gene was expressed under T7 promotor with a fusion partner of 6×His. Tag at its 5'terminal. After induction by 0.2mM IPTG for 18 h, the recombinant enzyme are expressing in the cytoplasm. The 1275 bp gene from P. acnes encode a linoleic acid isomerase protein with 424 amino acids (55KD).

Abstract: Implantation involves a complex process between the embryo and uterus and is crucial to further embryonic development. In this histochemical study, we investigated the early stage of embryo implantation and artificial decidualization in mice by the detection of total proteins and glycosaminoglycans using toluidine blue at pH 4.0 (TB) and Xylidine Ponceau at pH 2.5 (XP). TB staining showed mild metachromatic basophilia, which was more visible in the endometrial stroma around the implantation site in day 5 of pregnancy, and the level of glycosaminoglycans was more intense near the site of implantation on day 6, 8. Histochemical staining with XP was observed more intense in the stroma close to the site of implantation, especially in day 8 of pregnancy. At sites distant from the blastocyst were observed more discrete by staining with either TB or XP. Compared with TB, XP were more evident in the stroma around the uterine lumen under artificial decidualization. This study demonstrated changes in glycosaminoglycans and proteins by the detection of anionic and cationic radicals in the endometrial stroma adjacent to the site of embryo implantation during peri-implantation, post-implantation and artificial decidualization.

Abstract: The volitile oil was extracted with n-hexane and analyzed with GC-MS from the leaf, bark and heartwood of grafted Liquidambar orientalis Mill., and 22, 16, 18 compounds were identified respectively, of which γ-sitosterol was found in leaf, bark and heartwood, and 1-methoxy-3-(2-hydroxyethyl) was found in leaf and bark. Vitamin E was enriched in bark while dibutyl phthalate was abundant in heartwood.