Advanced Materials Research Vols. 641-642

Abstract: We reported here the gelation behaviors of binary trigonal acids and bipyridine derivatives mixtures in various organic solvents. Their gelation behaviors in 20 solvents were tested as new organic gelators. It was shown that the molecular shapes and substituent groups in these compounds played a crucial role in the gelation behavior of the compounds. While the trigonal acid with aromatic core can gel in acetone and ethanol, another trigonal acid with hydrophobic core can only gel in aniline. The molecular structures and substituent groups of bipyridine derivatives have also played an important role in changing the gelation behaviors and assembly states. SEM observations reveal that the molecular structures and the identity of the solvents are the main factors affecting the structures of the aggregates in the gels. Experimentally, different microstructures of the gels were observed. As example, the aggregates of trigonal compound in acetone, ethanol, or aniline adopt structures of belt-like aggregates or thin nanofibers, respectively.

Abstract: The growth of Bacillus subtilis was highly inhibited by crystal proteins of Bacillus thuringiensis ly36 strain with or without the treatment of proteinase K. And human hepatoma cell lines SMMC-7721 and Bel-7402 were killed by the proteinase K-treated crystal proteins of B. thuringiensis ly36 strain.

Abstract: Objective: Making a glucose sensor to detect the glucose which is extracted from the tissue fluid on reverse iontophoresis. Method: In the role of the catalysis of glucose oxidase which was fixed in polyethylene oxide gel, the glucose and potassium ferricyanide were change into gluconic acid and potassium ferrocyanide. Then we could get the concentration of glucose by detecting the current which was created by the redox reaction. Results: The glucose sensors could detect the concentration of glucose in the range of 2.2~22mmol/l and have a good linear too. The conformance test results show that the deviation of multiple measurements of the same sensor is less than 2% and the reaction time is less than 1s. Conclusion: The sensors could detect the blood glucose.

Abstract: The inducing effects of autoclavable IPTG and lactose solution on gene expression in genetically engineered bacteria were studied. Compared with the filter-sterilized one, the autoclavable IPTG and lactose solution of the same concentration can also show the same inducing effects respectively . All these mean that the IPTG and lactose solution used to induce gene expression in genetically engineered bacteria can be prepared more simply and more convenient so that the waste produced in the process of filter-sterilization can be prevented.

Abstract: Arachidonic acid (AA) is an essential component of structural lipids in the cellular membranes of mammals. To obtain efficient AA-producing microorganism, the strain of Mortierella isabellina 3410 was subject to mutagenesis by treating the wild-type spores exposed to ultraviolet or diethyl sulfate. A mutant of M. isabellina MS-1 was screened and chosen because of its higher AA production capability. Compared with the wild strain of M. isabellina 3410, the AA production by mutant of M. isabellina MS-1 was increased about 2.5 folds. Analysis by gas chromatography (GC) indicated that AA was the main fatty acid in the lipids extracted from the dried mycelia of the mutant of M. isabellina MS-1.

Abstract: Objective: The aim was to analyze Riemerella anatipestifer (RA) Cas1 gene and acquire more useful information for guiding the further study of the gene. Methods: Using bioinformatics method, sequence analysis of RA Cas1 gene was carried out with some databases and software. Results: Composition analysis of RA Cas1 gene, and homology search, sequence alignment, phylogenetic tree analysis and physicochemical property analysis of RA Cas1 protein were performed. Homology search revealed that Cas1 protein sequence of R. anatipestifer showed high similarity to those of strains within the Genus Capnocytophaga and Chryseobacterium, in accordance with the result of multiple sequence alignment and phylogenetic tree analysis. The rare codon analysis showed that there was little rare codon string in RA Cas1 gene. Conclusion: Cas1 gene of R. anatipestifer was evolutionally closely related to those of the Genus Capnocytophaga and Chryseobacterium, and can be over-expressed in E.coli without considering the impact of rare codons on translation.

Abstract: Nowadays the research for discovering SNPs is becoming one of the most important topics to understand the differences between genotypes of the same species. The objective of this study is to discover the SNPs using oligonucleotide microarrays in barley. Two methods are used for the prediction step: one is Robustified Projection Pursuit (RPP) method and another is based on a statistic test between probes of a probeset belonging different genotypes. Following the procedure described above, 124 single feature polymorphisms (SFPs), which can be used for identification of SNPs or insertion/deletions (INDELs), were detected among all genotypes. Sequencing of putative SFP-containing amplicons from the EST region in the genome yielded a validation rate of 85%, indicating the Affymetrix Barley 1 array could provide a satisfactory platform for identification of SNP in barley.

Abstract: In this paper, a class of HIV infection model with delayed immune response has been studied. We analyze the global asymptotic stability of the viral free equilibrium, and the stability and Hopf bifurcation of the infected equilibrium have been studied. Numerical simulations are carried out to explain the results of the analysis, and the change of the immune response of CTLs infects stability of system. These results can explain the complexity of the immune state of AIDs.

Abstract: The study established hyperlipemia rat model by high-fat diets feeding and high-fat solution gavage. Body weight and related bio-physiological parameters in plasma after 30 days of treatment were tested and then statistic analysis was performed. Body weight and bio-physiological parameters had significant difference in both high-fat diets feeding and high-fat solution gavage groups when they were compared with control group. Results of statistic analysis about two groups showed that the way of high-fat solution gavaging could reduce the influence on individual difference, meanwhile it could improve the uniformity and scientificity of the data compared with the other one.

Abstract: Method Endophytic Fungi were isolated from the stem, leaves and root of Angelica sinensis by the surface sterilization method and scoring method. the antimicrobial activity from isolated endophytic fungi were detected using filter paper method with Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae as indicator microorganisms. the partial isolates were identified based on morphological characteristics and classified according to ITS-rDNA sequences analysis. Result A total of 28 endophytic were isolated from the different tissues of the Angelica sinensis, antibiotic activity of the strain AS-R109 and AS-R203 against four indicator microorganisms were the highest, with inhibiting zones diameter of 18, 22, 25, 20 and20,22,19,23 mm respectively at concentration of 10mg/mL. With morphology and molecular biology identification, AS-R109 and AS-R203 was determined to belong to the genus for Alternaria sp. and Penicillium sp., respectively.