Advanced Materials Research Vols. 750-752

Abstract: t developed test devices and carried out test adopting combined process of clarification-adsorption-membrane separation targeted at surface water which was polluted by chemical agent VX. Investigation on purification effects of sand filtration, microfiltration, ultrafiltration, activated carbon adsorption and reverse osmosis was implemented in sections. It also researched on working principles of each section. Furthermore, comparative study has been made for adsorption performance of coaly granular activated carbon and that of shell activated carbon. The results show that the combined process can remove effectively simulation agent in water and the outlet quality complies with requirements of relevant standards.

Abstract: In inositol’s extraction process, the technological parameters and operational approaches of the following factors have a great influence on the final quality and yield: the quality and condition of raw material, acidolysis pressure, order of feeding and additives, use of neutralizer, removal of impurities in ion exchange section, vacuum degree in concentration section, evaporating temperature, outer steam pressure, purified washing and foreign ion tests. Though experiments, we concluded the key points and theoretical foundations to improve the products quality and yield. The results offer help for the actual production.

Abstract: The development history of biomedical titanium alloy was reviewed in this paper. Because of high recovery strain, low stiffness facilitating integration with bone structures and good market potential of porous biomedical material, the most common methods for fabricating porous biomedical material were introduced. The advantages and disadvantages of the methods were summarized from the aspects of process route, reaction mechanism and porosity. The main direction for further studies in this field was also suggested.

Abstract: Camptothecin (camptothecin, CPT) is a kind of anti-cancer drugs targeted at DNA topoisomerase with strong toxicity and side effects. The zebrafish is a model organism to study the anti-tumor drugs. The CPT was added into culture system in early development period of zebrafish embryos, and staining techniques were used to study embryonic development and angiogenesis in zebrafish. Results showed that low concentration of CPT can affect embryonic development seriously, and trigger the developmental delay which might led to teratism or death and accompanied with apoptosis phenomenon at the same time. Besides, CPT can also inhibit the development of intestinal vein in zebrafish embryos, which provided the experimental basis for the research of the pharmacological analysis, screening and evaluation model of topoisomerase I inhibitor class drugs.

Abstract: In this paper, we successfully designed a pH-responsive micelles based on hybrid polypeptide copolymers of poly (L-lysine-4-Azepan-1-yl-butyric)-b-poly (ethylene glycol)-b-poly (L-lysine-Diisopropylamide)-b-poly (L-leucine) (PLL(A)-PEG-PLL(B)-PLLeu) for efficient drug delivery. This pH-responsive nanoparticles were able to response to different pH values (pH=6.8 and 5.5). In vitro, these nanoparticles exhibited a stable and evenly distributed approximately 51 nm, a slightly positive potential about 10.3 mv at pH 7.4, which were crucial for the circulation of drugs in blood. While size and potential were about 130 nm and 34.7 mv at pH 6.8, which were good for drugs in membrane. Furthermore, the loading capability of DOX was up to 11.3%, and the pH-responsive release efficiency reached to 68.3% at pH 5.5. The results indicated that these micelles had huge potential application in cancer delivery.

Abstract: Arenga engleri is native to China, and widely distributed in southern China, which has a high economic value and development space. Through the initial test of arenga engleri fiber’s physical properties and chemical properties, we acquired the basic data and drew relevant conclusions.

Abstract: Supercritical fluid extraction (carbon dioxide), water extraction and ethanol extraction are used to extract bioactive components from fruiting bodies of Antrodia Camphorata to find out the optimum extraction condition through operation variable changes. Analysis comparisons tell that the best condition for water and ethanol approaches is 95% ethanol as the solvent, 45 °C as the operation temperature and 24 hours for extraction while supercritical fluid extraction prefers 95 % ethanol as the co-solvent, 250 bar as the working pressure and 45 °C as the operation temperature. There exist more and significant low polarity peaks in HPLC detection chromatograms for supercritical fluid extract liquids.

Abstract: Bifidobacterium lactis with oxalate-degrading capacity can efficiently reduce the oxalate in vivo, and it can be used to prevent and treat kidney stone diseases. While Bifidobacterium lactis is poorly oxygen-tolerant, which hinders it from being as microbial ecological agents. To obtain oxygen-tolerant and oxalate-degrading lactic acid bacteria, protoplast fusion technology was used between B. lactis and L. acidophilus. Under the optimum conditions of protoplast fusion with PEG 6000 concentration 50%, the fusion time 7 min, the fusion temperature 30°C, the concentration of CaCl₂ 0. 02mol/ L and the concentration of MgCl₂ 0.5mol/ L, the fusion rate reached 7.6%, and three oxygen-tolerant fusant showing that the level of oxalate degradation were similar with B. lactis was obtained. The fusants of SZY1-7 and SZY2-1 could tolerance to pH 2.5 and 0.5% (w/v) bile salt.

Abstract: In this paper, a novel dually-labeled DNA probe (DLP) in a stemloop structure was designed to be labeled at one end with dabcyl, working as a guest molecule, and at its another end with CdS nanoparticle, working as an electrochemical tag to indicate the electrochemical signal of hybridization occurrence. One a-cyclodextrin/multi-walled carbon nanotube modified glassy carbon electrode (a-CD/MCNTs/GCE) was fabricated for the hybridizer capture. The DLP could recognize target DNA through hybridization event between complementary bases in the loop of probe DNA and target DNA. Without binding with target DNA, the DLP kept its stemloop structure which held dabcyl molecule shielded by the loop of probe DNA and CdS nanoparticle. This structure blocked dabcyl from entering the cavity of a-CD on the electrode. Once hybridization happened, the stem-loop structure of hairpin DNA opened and then dabcyl molecule could be easily captured by a-CD on the electrode. Thus, the electrochemical response of the CdS nanoparticle label at the other end of the hybridizer was proportional with the concentration of target DNA.

Abstract: Protease was a key hydrolase in nitrogen transformation in landfill. The activity could show transformation states of nitrogen, that had important significance to study the biotransformation of organic nitrogenous substances in landfill. This paper designed six factors and five levels (L25 (56)) orthogonal test to measure the activity of protease in landfill. The results showed that the optimal experimental conditions for protease in landfill were 16h of culture period, 30°C of culture temperature, 6mL 1% casein, 0.3mL toluol, 0.2mL 0.1N H2SO4 and 1.5mL 20% Na2SO4. The experimental results were 84.23% to 368.86% more compared with that by the method of traditional method. In the optimized tests, the Relatively Standard Deviation (RSD) was less than 3.37%, and average recovery rates was 97.15%. The result showed the designed optimal experiments could provide more accurate and stable experimental data.