Advanced Materials Research Vols. 750-752

Abstract: The natural seleniferous rice protein peptide was prepared by coupling with mixed-enzymes and ultrasonic-microwave synergistic technology in this paper. The kinds of enzyme was atteined first of all. Then the mathematical model of correlation among enzyme dosage, extraction temperature, extraction time and liquid-solid ratio were established by means of orthogonal experimental design. The optimal extraction conditions were determined as follows, alkali protease dosage 2.0%, extraction temperature 50°C, extraction time 40 min and liquid- solid ratio 7:1(m:g). Under such conditions, the extraction ratio was 72.2%, Se content was 6.88 μg/ml. The method could significantly improve the yield of natural seleniferous rice protein peptide and reduce the extraction time .The rich selenium rice protein peptide provides a very good source of selenium way in order to solve the lack of selenium source.

Abstract: To improve the simulation accuracy of biodegradable polymer materials degradation model, a parameter optimization model was proposed, and the ant colony optimization algorithm (ACO) was used to solve the model and optimize undetermined parameters of the model. A comparative analysis of the calculation results was progressed which proved that the simulation accuracy of biodegradable polymer materials degradation of model can be significantly improved by using the optimized parameters. This parameter optimization is propitious to demonstrate the science meaning of degradation mechanization and guide the design and product of medical devices.

Abstract: In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pUC19-His expression vector, then the recombinant expression vector was transformed into host bacteria E. coli BL21. The recombinant chicken IFN-α was induced to express by IPTG, then the protein expression was analyzed with SDS-PAGE. Under the condition that the recombinant protein was induced to express with 1 mM IPTG at 37 °C, the expressed protein was inclusion body. His-chIFN-α was purified by Ni-metal chelate affinity chromatography. His-chIFN-α was shown to inhibit the replication of Newcastle disease virus in CEF cells.

Abstract: The effect of chelating agents Diethylene Triamine Penta Methylene Phosphonic Acid in bamboo kraft cooking was studied. The results show that the bamboo pulp yield was 49.52% when DTPMPA dosage is 0.4%, at the same time the Kappa number was lower. With the increasing of DTPMPA dosage in bamboo kraft pulping, the bamboo pulp strength was increased.

Abstract: In the present study, we investigated the anti-fatigue activity in male Kunming mice with pecan oil using a forced swimming test. Mice were divided into four groups (three pecan oil administered groups and a control group). The control group was gavaged with distilled water and pecan oil administered groups were gavaged with pecan oil (10, 20 and 30mg/10g body weight /d). After four weeks, a forced swimming test was performed and the biochemical parameters related to fatigue were examined. The results suggested that pecan oil could extend the swimming time to exhaustion of the mice, as well as increase the tissue glycogen contents, while decreasing the blood lactate and serum urea nitrogen contents. This indicated that pecan oil had anti-fatigue activity and could elevate the exercise tolerance.

Abstract: To explore the protective effects of salidroside against endogenous hydrogen peroxide (H₂O₂) -induced cytotoxicity in human endothelial cells (EVC-304). EVC-304 cells were incubated in the presence or absence of low steady states of H₂O₂ (34μM) generated by glucose oxidase (GOX) with or without salidroside. MTT assays were performed, together with flow cytometric analysis using propidium (PI) label. The results indicated that salidroside could attenuate H₂O₂ induced cytotoxicity in EVC-304 cells in a dose-dependent pattern. Furthermore, flow cytometric analysis revealed that salidroside could also inhibited the G2/M arrest induced by endogenous hydrogen. The present study demonstrates that salidroside could inhibit endogenous hydrogen peroxide induced cytotoxicity of endothelial cells .

Abstract: We assessed the effectiveness and mechanism of action of schisandrin on modulation of learning and memory disorders in mice. Memory impairment was established in mice by intraperitoneal injection of pentobarbital sodium (20 mg/kg). Schisandrin (0.5, 1.0, 2.0g/kg) were administered by intragavage once daily for 14 consecutive days. The Morris water maze test was used to evaluate the ability of schisandrin to reduce phenobarbital-induced learning and memory impairment. The levels of superoxide dismutase (SOD) nitric oxide (NO) and catalase (CAT) were measured in brain tissue samples taken from the mice. Other biomarkers measured included expression of nuclear transcription factor-kappa-B (NF-κB) and brain-derived neurotrophic factor (BDNF) in the hippocampus CA1 region, which were determined by immunohistochemical analysis. On the fifth day of treatment, the mice in the pentobarbital sodium group performed worse on the Morris water maze test compared to untreated controls (P<0.01), which could be prolonged after schisandrin treatment (P<0.05 for="" low="" and="" intermediate="" dose="" groups="" analysis="" of="" brain="" tissues="" showed="" that="" compared="" with="" the="" control="" group="" no="" levels="" were="" increased="" sod="" cat="" activity="" decreased="" in="" pentobarbital="" sodium="" i="">P<0.01). After treatment with schisandrin, the NO levels were significantly decreased (P<0.01), while SOD and CAT activity increased (P<0.01). Immunohistochemistry analysis showed that, in phenobarbital only group, the protein expression of BDNF decreased, NF-κB increased compared to untreated controls, and schisandrin could reverse this trend (P<0.05 and="" i="">P<0.01, respectively). The results suggest that schisandrin is effective in improving the learning and memory deficiency induced by pentobarbital sodium, the mechanism of which may be related modulation of cellular response to oxidative stress.

Abstract: This paper presented a novel heating technique of biomass carbonization system using microwave radiation. The heating system employs multi-feed microwave generators for uniform heating distribution without stirrer mechanism in a 0.847 m³ cylindrical low cement castable reactor. The experiment entailed the carbonization of 3,500 kg of coconut shell at 450°C for 10 hr. Afterward, the charcoal and wood vinegar yields were analyzed; meanwhile, uncondensed gases were treated to fuel the engine-generator system. The results show that the proposed approach greatly saves the production time of charcoal yields and allows ease of control.

Abstract: In this study, schisandrin was assessed for potential protective effects on pheochromocytoma cell line (PC12 cells). Using PC12 cells damage induced by H₂O₂(25μmol/L) as in vitro model. After pre-treatment with different concentration of schisandrin (0.3, 0.6, 1.2μM) for 24h, MTT assay was used to detect the cell viability, the supernatant of cells was collected to examine the levels of nitric oxide (NO) in each sample, and immunocytochemical staining was adopted to observe the expression levels of bcl-2. Results showed that schisandrin at different concentrations could increase the viability of PC12 cells and decrease the levels of NO in the culture medium. There were significant differences between schisandrin group and H₂O₂ group (P<0.05,P<0.01). Immunocytochemical staining result revealed that schisandrin could upregulate bcl-2 protein expression. In summary, schisandrin shown significant neuroprotective effects on H₂O₂-injured PC12 cells through antioxidant properties and upregulate bcl-2 protein expression, and could be a potential candidate for intervention in neurodegenerative diseases.

Abstract: Objective: To investigate the effect of Radix playtycodoni polysaccharide (RPPS) on rats with ovalbumin-induced asthma and its mechanisms. Methods: 48 Wistar rats were randomly divided into blank control group, asthma model group, dexamethasone control group (1 mg / kg), and high-dose RPPS group (40 mg / kg), moderate-dose RPPS group (20 mg / kg) and low-dose RPPS group (10 mg / kg). The ovalbumin sensitization method was used to establish the asthma model in rats. The rats were given it in the atomization administration for two weeks and then were sacrificed. The BALF was collected for the count of eosinophils (EOS); enzyme-linked immunosorbent assay (ELISA) method was employed for the determination of IL-4, IL-5, and IFN-γ levels in BALF; the expression of NF-κВ in the lung tissues was measured by Western blot. Results: Compared with the model group, RPPS could reduce the number of EOS in blood and BALF, increase the content of IFN-γ in BALF, lower IL-4 and IL-5 contents, and down-regulate the expression of NF-κВ protein in rats with asthma. Conclusion: RPPS can inhibit the airway inflammation in asthma, down-regulate the expression level of IL-4 and IL-5, up-regulate the IFN-γ level to regulate the abnormal immune status induced by the imbalance of TH1/TH2 in asthma and inhibit the expression of NF-κВ, which may be one of the mechanisms of its anti-inflammatory activity.