Paper Titles

Effects of Cu and K Promoters on the Catalytic Performance of Iron-Based Nanocatalyst for Fischer-Tropsch Synthesis
p.15

Investigation on the Morphology of Sintered Silver Nanomaterial for Electronic Packaging Application
p.21

Effect of Processing Route on the Morphology of Thermoplastic Polyurethane (TPU) Nanocomposites Incorporating Organofluoromica
p.27

Effect of Parameters on Lattice Thermal Conductivity in Germanium Nanowires
p.33

Cell Traction Force Mapping in MG63 and HaCaTs
p.39

Influence of Nanoparticle Type, Size and Weight on Migration Properties of Nanorefrigerant
p.45

Effect of Annealing on Surface of Nickel (Ni)/Indium Tin Oxide (ITO) Nanostructures Measured by Atomic Force Microscopy (AFM)
p.51

A Study on the Effect of Calcination Temperature on the Graphitization of Carbon Nanotubes Synthesized by the Decomposition of Methane
p.56

Formation of Carbon Nanotubes from Methane Decomposition: Effect of Concentration of Fe₃O₄on the Diameters Distributions
p.62

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 832Cell Traction Force Mapping in MG63 and HaCaTs

Cell Traction Force Mapping in MG63 and HaCaTs

Article Preview

Abstract:

The ability of a cell to adhere and transmit traction forces to a surface reveals the cytoskeleton integrity of a cell. Shear sensitive liquid crystals were discovered with new function in sensing cell traction force recently. This liquid crystal has been previously shown to be non-toxic, linear viscoelastic and sensitive to localized exerted forces. This paper reports the possibility of extending the application of the proposed liquid crystal based cell force sensor in sensing traction forces of osteoblast-like (MG-63) and human keratinocyte (HaCaT) cell lines exerted to the liquid crystal sensor. Incorporated with cell force measurement software, force distributions of both cell types were represented in force maps. For these lowly contractile cells, chondrocytes expressed regular forces (10 – 90 nN, N = 200) around the circular cell body whereas HaCaT projected forces (0 – 200 nN, N = 200) around the perimeter of poly-hedral shaped body. These forces are associated with the organisation of the focal adhesion expressions and stiffness of the LC substrate. From the results, liquid crystal based cell force sensor system is shown to be feasible in detecting forces of both MG63 and HaCaT.

You might also be interested in these eBooks

Nanoscience, Nanotechnology and Nanoengineering

Info:

Periodical:

Advanced Materials Research (Volume 832)

Pages:

39-44

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.832.39

Citation:

Cite this paper

Online since:

November 2013

Authors:

Chin Fhong Soon, Mohamad A. Genedy, Mansour Youseffi, Morgan C.T. Denyer

Keywords:

Cell Force Sensor, Cell Traction Force, Keratinocytes, Liquid Crystal, Osteosarcoma

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] K. Burridge, M. Chrzanowska-Wodnicka, Focal adhesions, contractility and signaling, Annu. Rev. Cell Dev. Biol., 12 (1996) 463-519.

DOI: 10.1146/annurev.cellbio.12.1.463

[2] C.F. Soon, M. Youseffi, R.F. Berends, N. Blagden, M.C.T. Denyer, Development of a novel liquid crystal based cell traction force transducer system, Biosens. Bioelectron., 39 (2013) 14-20.

DOI: 10.1016/j.bios.2012.06.032

[3] C.F. Soon, M. Youseffi, N. Blagden, R. Berends, S.B. Lobo, F.A. Javid, M. Denyer, Characterization and biocompatibility study of nematic and cholesteryl liquid crystals in: Proc. WCE, 2009, pp.1872-1875.

[4] C.F. Soon, M. Youseffi, N. Blagden, M. Denyer, Effects of an enzyme, depolymerization and polymerization drugs to cells adhesion and contraction on lyotropic liquid crystals, Proc. WCE, 1 (2010) 556-561.

[5] C.F. Soon, N. Nayan, M. Youseffi, N. Blagden, M.C.T. Denyer, Effects of Trypsin and Cytochalasin-B Treatments to Cell Traction Forces, in: P.D.F. Ibrahim (Ed.) International Conference of Biomedical Engineering and Sciences, IEEE-EMBS, Langkawi, Malaysia, 2012.

DOI: 10.1109/iecbes.2012.6498084

[6] C.F. Soon, M. Youseffi, N. Blagden, M. Denyer, Measurement and mapping of cell traction forces on liquid crystal based force transducer, in: International conference on computational biosciences, IASTED, Cambridge university, 2011.

DOI: 10.2316/p.2011.742-013

[7] H. Declercq, N.V.d. Vreken, E.D. Maeyerb, R. Verbeeck, E. Schacht, L.D. Ridder, M. Cornelissen, Isolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source, Biomaterials, 25 (2004) 757-768.

DOI: 10.1016/s0142-9612(03)00580-5

[8] H. Watanabe, C.A. Mackay, E. Kislauskis, A. Mason-Savas, S.C. Marks Jr., Ultrastructural evidence of abnormally short and maldistributed actin stress fibers in osteopetrotic (toothless) rat osteoblasts in situ after detergent perfusion, Tissue Cell, 29 (1997) 89-98.

DOI: 10.1016/s0040-8166(97)80075-4

[9] A.J. Singer, A.F. Richard, A.F. Clark, Cutaneous wound healing, The New England Journal of Medicine, (1999) 738-746.

[10] P. Boukamp, R. Petrussevska, D. Breitkreutz, J. Hornung, A. Markham, N. Fusenig, Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line, J. Cell Biol., 106 (1988) 761-771.

DOI: 10.1083/jcb.106.3.761

[11] M. Takeo, Skin biomechanics from microscopic viewpoint: mechanical properties and their measurement of horny layer, living epidermis, and dermis, Fagr. J., 35 (2007) 36-40.

[12] F.M. Hendriks, Mechanical behaviour of human epidermal and dermal layers invivo, Technische Universiteit Eindhoven, Eindhoven, 2005.

[13] A.D. Bershadsky, N.Q. Balaban, B. Geiger, Adhesion-dependent cell mechanosensitivity, Annual Review of Cell Developmental Biology, 19 (2003) 677-695.

DOI: 10.1146/annurev.cellbio.19.111301.153011

[14] B. Geiger, A. Bershadsky, Exploring the neighborhood: adhesion-coupled cell mechanosensors, Cell, 110 (2002) 139-142.

DOI: 10.1016/s0092-8674(02)00831-0

[15] S. Tojkander, G. Gateva, P. Lappalainen, Actin stress fibers-assembly, dynamics and biological roles, J. Cell Sci., 125 (2012) 1-10.

DOI: 10.1242/jcs.098087

[16] R.I. Sharma, J.G. Snedeker, Paracrine interactions between mesenchymal stem cells affect substrate drive differentiation toward tendon and bone phenotypes, PLoS One, 7 (2012) e31504.

DOI: 10.1371/journal.pone.0031504

[17] A.J. Engler, M.A. Griffin, S. Sen, C.G. Bönnemann, H.L. Sweeney, D.E. Discher, Myotubes differentiate optimally on substrates with tissue-like stiffness pathological implications for soft or stiff microenvironments, Cell. Biol., 166 (2004b) 877-887.

DOI: 10.1083/jcb.200405004

[18] J.H.-C. Wang, J.-S. Lin, Cell traction force and measurement methods, Biomech. Model. Mechanobiol., 6 (2007) 361-371.

DOI: 10.1007/s10237-006-0068-4

[19] J. Stanley, P. Hawley-Nelson, M. Yaah, G.R. Martin, S. Katz, Laminin and bullous pemphigoid antigen are distinct basement membrane proteins synthesized by epidermal cells, J. Invest. Dermatol, 78 (1982) 456-459.

DOI: 10.1111/1523-1747.ep12510132

[20] E.A. O'Toole, Extracellular matrix and keratinocyte migration, Clin. Exp. Dermatol., 26 (2001) 525-530.

[21] W. Li, G. Henry, J. Fan, B. Bandyopadhyay, K. Pang, W. Garner, M. Chen, D. Woodley, Signals that initiate, augment, and provide directionality for human keratinocyte motility, J. Invest. Dermatol, 123 (2004) 622-633.

DOI: 10.1111/j.0022-202x.2004.23416.x

[22] A. Engler, L. Bacakova, C. Newman , A. Hategan, M. Griffin, D. Discher, Substrate compliance versus ligand density in cell on gel responses, Biophys. J., 86 (2004a) 617-628.

DOI: 10.1016/s0006-3495(04)74140-5

[23] K. Owaribe, R. Kodama, G. Eguchi, Demonstration of contractility of circumferential actin bundles and its morphogenetic significance in pigmented epithelium in vitro and in vivo, Cell. Biol., 90 (1981) 507-514.

DOI: 10.1083/jcb.90.2.507

[24] P. Hotulainen, P. Lappalainen, Stress fibers are generated by two distinct actin assembly mechanisms in motile cells, J. Cell Biol., 173 (2006) 383-394.

DOI: 10.1083/jcb.200511093