Galactosylated Chitosan/Carbonate Apatite Nanohybridization for Cell Specificity and High Transfection Efficiency as a DNA Carrier

In Young Park; Tae Hee Kim; You Kyoung Kim; Yun Jaie Choi; Jae Woon Nah; Chong Su Cho

doi:10.4028/www.scientific.net/KEM.342-343.437

Paper Titles

Aqueous Solubilization of Paclitaxel Using Hydrotropic Polymer Micelle
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A Poly(β-Amino Ester) of Spermine and Poly(ethylene Glycol) Diacrylate as a Gene Carrier
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Release of All-Trans Retinoic Acid (RA) from RA-Loaded Poly(Ester Amine) Based on Polyethylenimine and Polycaprolactone for Intracellular Delivery
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Alginate-Coated Thiolated Chitosan Microspheres for an Oral Drug Delivery System In Vitro
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Galactosylated Chitosan/Carbonate Apatite Nanohybridization for Cell Specificity and High Transfection Efficiency as a DNA Carrier
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PEGylation of Conjugated Linoleic Acid and its Application as an Anti-Cancer Prodrug
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Thiolated Eudragit-Coated Chitosan Microspheres as an Oral Drug Delivery System
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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo
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Novel Poly(Ester Amine) Based on Polycaprolactone and Polyethylenimine as a Gene Carrier: Effect of Hydrophobicity on Transfection Efficiency and Cytotoxicity
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Galactosylated Chitosan/Carbonate Apatite Nanohybridization for Cell Specificity and High Transfection Efficiency as a DNA Carrier

Abstract:

The strategies developed for gene delivery are generally classified into two categories of viral and non-viral vectors. The limitation of viral vectors, which have problems including toxicity, immunogenicity and inflammatory response has led to the development of a novel, synthetic vectors based on non-viral vectors. Chitosan, one of non-viral vectors, has been a good candidate in gene delivery field. Moreover, galactosylated chitosan (GC) had the specific recognition of hepatocytes by galactose in the GC. Also, carbonate apatite increased the rate of DNA endocytosis and the efficiency of gene transfer. We describe here a new concept for improving cell specificity and transfection efficiency by hybridization of carbonate apatite (CAp) with GC. The complex formation was confirmed by agarose gel electrophoresis. The complex optimized through controlling calcium ion and charge ratio was evaluated on the cell specificity and transfection efficiency.