Advanced Materials Research Vols. 393-395

Abstract: A novel functional material was fabricated by combining levofloxacin microspheres with viscose nonwoven fabrics. The complex microspheres were made by w/o emulsion crosslinking technique. The morphologies of both microspheres and fabric composite were investigated by SEM. The drug release property was evaluated by UV. The antibacterial activity and cytotoxicity were tested by spread plate and cell culture method. The results showed that the microspheres had spherical shape and could be embedded into the viscose fabrics. The levofloxacin was released gradually within 168 hours and its release kinetics corresponded to diffusion-relaxation model. In addition, the antibacterial activity of the fabric was increased with prolonged drug releasing time and the complex fabrics had no obvious cytotoxicity. The functional material has great potential to be used as a wound dressing with antibacterial property.

Abstract: The objective of this study was to understand the effect of biomass concentration (BC), milling times (MT) and volumetric hexane to water ratio (HWR) on lipid recovery rate of microalgae Scenedesmus dimorphus in the wet milling process. A second-order response surface regression model (R²>95%) was developed based on a three-variable five-level experiment using the Central Composite Design method. Modeling results indicated that HWR (Pr<0.001) and BC (Pr<0.01) significantly affected lipid recovery rate. The optimum settings were found to be 21.2 g l^-1 BC, 4 MT and 0.70 HWR, at which 98.5% of lipids were recovered. The results indicated that the wet grinding mill was effective in lipid recovery from S. dimorphus.

Abstract: Using the Siemens PLC programmable controller, a new weighing and control integration plan was discussed, and the debugging points was analyzed according to the engineering experience, and the principle part and program details were presented.

Abstract: In this study, the metabolic network of ectoine by Halomonas venusta DSM 4743 was established. The key nodes to influence the ectoine fermentation in metabolic flux and the basis during optimal control of fermentation process were investigated. The results showed that G6P, α-KG and OAA nodes were the key factors to influence the synthesis of ectoine. The metabolic flux distributions at the key nodes were significantly improved and ectoine concentration was enhanced in ectoine fermentation by adopting monosodium glutamate as the sole carbon and nitrogen sources, feeding monosodium glutamate and supplying oxygen limitedly. The batch fermentation was carried out in 10 L fermentor , the concentration and yield of ectoine was 8.4 g/L and 0.1 g/g, respectively, which were increased by 2.8 and 2 times, by comparison with batch fermentation using glucose as carbon source.

Abstract: Xylanse named as Pulpzyme HC was used to modify bleached softwood pulp before refining. The efficiency of modification by different enzyme dosage was invested in this paper. The result showed that enzymatic treatment decreased the dissolved charge and absolute Zeta potential of the slurry and increased the fiber surface wettability. Appropriate Pulpzyme HC (0.4u/g) could improve the refining efficiency by 9.5°SR. The brightness and bulk of hand sheets increased with the increasing of the enzyme. Tensile index and tear index of hand sheets reached the maximum at the enzyme dosage of 0.2u/g. In this dosage, fiber length, knot index and curl index of fiber increased. But excessive enzyme decreased the tear index and had no effect on the tensile index.

Abstract: Agaricus brunnescens is a producer for novel biomaterial (exopolysaccharide of Agaricus brunnescens). In this paper, the optimization of culture conditions for the biomass of Agaricus brunnescens through orthogonal methods was reported. The optimal conditions for enhancing production was: inoculum size 10 %, broth volume 100 mL in a 250 mL flask, pH 6.0. This optimization strategy in shake flask culture lead to an biomass 0.8 g/L, which was considerably higher than that obtained in preliminary studied.

Abstract: In order to investigate the role of a rice NAD-malic enzyme gene (OsNAD-ME1) under different abiotic stresses, OsNAD-ME1 was constructed into plant expression vector and transformed into Agrobacterium for infecting Arabidopsis thaliana. There were higher transcriptional levels of OsNAD-ME1 in homozygous transgenic lines compared with WT plants as well as higher NAD-ME activity. WT and transgenic Arabidopsis were treated with NaCl, NaHCO₃, mannitol and H₂O₂. And then their root lengths, crown widths and fresh weights were measured and compared. The results showed that over-expression of OsNAD-ME1 in transgenic Arabidopsis increased the resistance to abiotic stresses, which indicated that OsNAD-ME1 was related to stress tolerance.

Abstract: In order to save electric energy for classroom lighting in universities, energy-saving controllers are used to control turning-on and turning-off of lights in a classroom based on the illumination intensity and the usage conditions of the classroom. Three parameters for an energy-saving controller, namely turning-on threshold, turning-off threshold, and delaying time, should be set before usage, which is usually done based on a user’s experience. In this paper, for optimizing the parameters, a multi-objective optimization model for an energy-saving controller is established. Then, fuzzy logic is used to optimize the parameters. In this way, an energy-saving controller can be used well.

Abstract: Fructus Cnidii (FC) is a traditional Chinese medicine, but it has mild toxicity. To reveal mechanism of FC toxicity, we carried out metabonomics study. We exported mass data to be processed by chemometrics methods with R. Pattern recognition ability of different methods in different packages were evaluated. The result showed OPLS has powerful discriminative ability of pattern recognition. This work laid the foundation for selecting biomarkers of FC toxicity to reveal it’s mechanism.

Abstract: Kuerle fragrant pear (Pyrus serotina Reld) were stored in different controlled atmospheres (4-6 % O2 plus 2-4 % CO2) and air control at -1~0 °C to determine the effects of different O2 and CO2 atmospheres on physiology, quality and decay during storage. The results indicated that CA-I inhibited polyphenol oxidase (PPO) activity, reduced total phenol content, prevented peel browning and decreased fruit decay in comparison with CA-II and air control at -1~0 °C, and more effectively reduced ethanol production in the flesh and maintained peel green color and extended storage life of Kuerle fragrant pear by comparison with CA- II treatment and air control at -1~0 °C treatment.