Advanced Materials Research Vol. 790

Abstract: Coaxial electrospinning technique was developed on the basis of electrospinning. Despite the common advantages of electro-spun, it can also protect the additives which were covered in the nuclear of the yarn during the spinning process and control the release of these additives, such as drugs, proteins, magnetic particles and the active agent, moreover it can be made in one-step. These make Coaxial electro-spun nanofibers have a broad application prospects in the field of wound dressings and drug release. This paper is to describe the unique applications of coaxial electro-spinning in wound healing and the further research of this technology.

Abstract: As a classical method for sulfide determination in wastewater, iodimetry is adopted by many countries. But the existing of high concentration of reducing materials such as thiosulfate, sulfite and dissolved organic, which cant be eliminated by the pretreatment of zinc acetate-precipitation filtration, have an important influence on determination. The study on elimination method is carried out in this paper. It is indicated that the interference of S₂O₃^2- and SO₃^2- can be eliminated by washing sedimentation with KOH solution of 10%. Iodometric titration with the pretreatment of zinc acetate-precipitation filtration and washing precipitate with 10% KOH is an accurate method to determine the sulfide content.

Abstract: This system essentially implements the information from RS and GIS associated with malaria epidemic modeling, and increases precision of malaria epidemic modeling by the information representing physical and logical fusion objects subject to malaria epidemic monitoring. This work focuses on the system architecture model and related design issues, including area and type estimation of malaria epidemic, whereas we use the model as a tool of data fusion to avoid the parameter estimation problems in complex environments.

Abstract: This paper addressed the problem of improving precision of malaria epidemic remote sensing by developing optimum image-fusion system, which analyses the implementation of image-fusion system through interpretation-lattice and takes into account that the benefits of image-fusion are maximized and the risk of error malaria epidemic recognition with remote sensing is minimized. We tested our RS image-fusion method with an application about monitoring malaria epidemic that is based on several TM and SPOT images and local statistical data. This method is better able to estimate the malaria epidemic level in comparison with only one single TM or SPOT image, the main method that was previously applied in this context.

Abstract: Objective To discuss the role of Beclin1 gene in autophagy and apoptosis induced by ionizing radiation in MCF - 7 cells. Methods MTT assay was used to detect the influence of cells proliferation when beclin-1 gene was over expression and interference. Flow cytometric analysis detected the change of MCF - 7 cells apoptosis after irradiating by X-ray. Western blot method detected total protein changes of beclin - 1. Results Beclin-1 gene was interferred partly, the amount of protein expression of MCF-7-beclin1Ri cells reached to the lowest at 4 h, rose at 8 h, got to the most at 16 h and decreased at 32 h, but still held at higher level;Beclin-1 gene over-expression, the amount of protein expression increased gradually with the extension of time, and got to peak at 32 h. Conclusions Ionizing radiation can stimulate beclin-1 and exist relations of dose - effects in MCF-7 cells.

Abstract: Type 2 diabetes was induced by feeding with high-sucrose-high-fat diet and injecting 25mg/kg streptozotocin. Rats were randomly divided into three groups: control, diabetic, diabetic simultaneously injected ip with Ginkgo biloba extract (EGb) for 8 wk. The treatment of EGb attenuated the development of hyperglycemia and hyperinsulinemia in type 2 diabetic rats. The serum levels of cholesterol, triglyceride as well as the activities of alanine aminotransferase, aspartate aminotransferase were significantly decreases in EGb-treated rats. The level of malondialdehyde were significantly increased in diabetic rats and decreased after EGb administration. Moreover, EGb markedly increased the activities of superoxide dismutase, catalase and glutathione peroxidase in liver. The results indicate that EGb has a protective effect on liver of type 2 diabetic rats, which may be related to decreases the accumulation of cholesterol, triglyceride in liver and its antioxidant property.

Abstract: To study the effect of chelidonine on Cdk1, p-Cdk1( Thr14), cyclinB1 protein expression in SGC-7901 Cells, and to explore the mechanism of chelidonine inducing G₂/M phase arrest in SGC-7901 Cells. Western Blotting was used to study the effects of chelidonine on the Cdk1, p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells. Chelidonine could significantly decrease the expression of Cdk1 and cyclinB1 protein. Meanwhile, chelidonine could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner. Chelidonine can down-regulate the expression of Cdk1, cyclinB1 and up-regulate p-Cdk1 (Thr14) protein expression in SGC-7901 cells,which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 cells which may be one of the role mechanism of G₂ /M phase arrest induced by chelidonine in SGC-7901 Cells.

Abstract: Food preservatives could cause a wide spectrum of antibacterial efficacies against different spoilage microbial species or strains; however, the mode of variation in antibacterial activities among and within species is still unclear which limits their effective applications in food preservation. In this study, ten thermoresistant spore-forming Bacillus strains as a typical spoilage microbial group were used to evaluate the variances of antibacterial activities of three chemical and three biological food preservatives among these species or strains by the statistical analysis of MIC using SAS 9.2 software. The biological preservatives exhibited much higher anti-bacillus activities compared to the chemical preservatives, and significant variances of MICs were observed among different Bacillus species for chemical preservatives while no variances were observed for biological preservatives. Variances of MICs among different strains in a species were not observed for both chemical and biological preservatives. The possible antibacterial mechanism has also been discussed to encounter food spoilage.

Abstract: The purpose of this study is to investigate the effect of fucosterol on the induction of apoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells. HL-60 Cells were treated with different concentrations of fucosterol at different time. MTT method was used to study fucosterol anti-tumor activity. Morphology observation was performed to determine the effects of fucosterol on apoptosis of HL-60 cells. Flow cytometry (FCM) was used to detect the cell cycle. Laser scanning confocal microscope (LSCM) was used to analyze the expressions of Fas, FasL, Fadd and Caspase-8. Caspase activity kits were used to determine the activity of Caspase-8 and Caspase-3. The results showed fucosterol could inhibit the growth of HL-60 cells, and the apoptosis morphology for 48 h treatment was obvious, which showed cell protuberance, cytoplasm concentrated and apoptotic body. Fucosterol treatment for 24 h increased the protein expression of Fas, FasL, Fadd and Caspase-8. It also showed that the activity of Caspase-3 and Caspase-8 has increased significantly. In conclusion, Fucosterol could induce HL-60 cells apoptosis via death receptor pathway.

Abstract: The purpose of this study is to investigate the effect of fucosterol on the induction of apoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells. HL-60 Cells were treated with different concentrations of fucosterol at different time. MTT method was used to study fucosterol anti-tumor activity. Morphology observation was performed to determine the effects of fucosterol on apoptosis of HL-60 cells. Flow cytometry (FCM) was used to detect the cell cycle. Laser scanning confocal microscope (LSCM) was used to analyze mitochondrial membrane potential (MMP). Western blot was performed to analyze the expressions of Cyt-C, Caspase-9 and Caspase-3. The results showed fucosterol could inhibit the growth of HL-60 cells, and the apoptosis morphology for 48 h treatment was obvious, which showed cell protuberance, cytoplasm concentrated and apoptotic body. Fucosterol treatment for 24 h decreased MMP in dose-dependent manners. It also induced the release of Cyt-C and the activation of Caspase-9 and-3. In conclusion, Fucosterol could induce HL-60 cells apoptosis through a mitochondrial pathway.