Quick and Sensitive Detection of Prion Disease-Associated Isoform (PrP^Sc) Using a Novel Gold Surface/PrP^Sc/Gold Nanoparticles Sandwich SPR Detection Assay

Abstract:

Prion protein has drawn great attention due to its pathological potential to prion diseases. Discriminate and detection of the trace quantities PrP^Sc is an important measure for prion disease diagnosis at the presymptomatic stage. In this study, we developed a novel sandwich surface plasmon resonance (SPR) assay for the detection of PrP^Sc, involving bare gold surfaces and bare gold nanoparticles. PrP^Sc can be captured by the SPR sensing surface via a surface assisted coupling reaction between its intra-molecular disulfide bond and the gold atoms, while PrP^C cannot bind to the gold surface strongly. The gold nanoparticles were proved to amplify the SPR detection signals via the coupling of their localized surface plasmon (LSP) with the propagating plasmon on the SPR gold surface. Our results confirmed that the bare SPR gold surface successfully captured the PrP^Sc from the solution with a LOD of 0.5ng/mL and a linear detection range from 0.5ng/mL to 500ng/mL. Injecting the gold nanoparticles after PrP^Sc yielded a dramatic enhancement of signal, with a lower LOD of 0.001ng/mL and a linear detection range from 0.001ng/mL to 10ng/mL. The gold nanoparticles permitted 4 to 322-fold increase of the signals. The required detection time was controlled in 15 min. PrP^C, cys-protein G and their mixtures with PrP^Sc, were also detected via this sandwich SPR detection assay. Atomic force microscope (AFM) was used to evaluate the surface morphology of the SPR gold substrate after the detection. All the obtained results suggested that this novel SPR sandwich detection assay in our work was efficient, sensitive and specific for the detection of trace PrP^Sc

• Periodical: Journal of Nano Research (Volume 48)
• Pages: 18-28
• DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.48.18
• Online since: July 2017
• Authors: Zhi Chao Lou*, Jin Qiang Sun, Jin Feng Wan, Xiao Hong Zhang, Hai Qian Zhang, Ning Gu
• Keywords: Gold Nanoparticle, Prion Protein, Sandwich Detection Assay, Signal Amplification, Surface Plasmon Resonance

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