A Simple Method for Depositing DNA on the Mica

Abstract:

For studying DNA electrical double layer in electrolyte solution, the key process is to create the template for DNA processing. In this paper, we described a straightforward method for depositing DNA on a substrate. The mica was chosen as the substrate materials because of its atomically flat and negatively charged surface in solution, and mica was coated by the polylysine to absorb the DNA molecules. Using by AFM, the DNA molecules have been probed obtaining a high resolution in air and liquid, and the method is simple and easy operated. This method can be used for nano-manipulation and the quality of the DNA under physiological conditions.

• Periodical: Key Engineering Materials (Volume 503)
• Pages: 145-150
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.503.145
• Online since: February 2012
• Authors: Yu Zhang Wang, Jing Jie Sha, Lei Liu, Hong Yan Fu, Yun Fei Chen
• Keywords: Atomic Force Microscope (AFM), DNA, Mica, Polylysine

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