Advanced Materials Research Vols. 655-657

Abstract: Purpose: To assess determination of Bacillus Calmette-Guerin(BCG) concentration integrated in delivery materials for intravesical infusion therapy of superficial bladder cancer. Material and Methods: The standard BCG solution was prepared and divided into two groups. One group was measured by spectrophotometer direct, the other group was determined by using enzyme mark instrument after the solution had cultured. The preliminary experiment study on BCG integrated delivery materials was finished by XTT method. Results: The wavelength of the characteristic peak changes with the variety of the concentration measured by spectrophotometer direct. The concentration of BCG which was embeded in integrated delivery materials for intravesical infusion therapy of superficial bladder cancer was linear with absorbance at 450nm by XTT method in the selected range. Conclusions: It is an incorrect way to measure BCG concentration like determining other solutions by the spectrophotometer immediately and direct. It is the simple, rapid and reliable method by XTT method to study on the performance of the BCG concentration integrated in delivery materials for Intravesical infusion therapy of superficial bladder cancer.

Abstract: 120 crucian carp were divided into 4 groups. Each group included two repeats with each of 15 crucian carp, and was fed with feedstuff mixed probiotics concentrations at 0.0%, 0.2%, 0.4%, and 0.6%. The figure index and viscera index were measured, and the nutrient compositions were analyzed after 32 feeding days. As a result, the best probiotics concentration is 0.4% on the increase of the weight gain and the nutrient compositions.

Abstract: The presence of crystallites in urine is closely related to stones formation. In this article, the components, morphology of nano- and micro-crystallites in urines of 20 uric acid (UA) stone formers as well as their relationship with the formation of UAstones were comparatively studied using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The main constituent of urinary crystallites was uric acid. Their particle size distribution was highly uneven, ranging from several nanometers to several tens of micrometers, and obvious aggregation was observed. These results showed that there was close relationship among stone components, urinary crystallites composition and urine pH.

Abstract: The interaction mechanism of a series of fentanyl analogs are examined using molecular docking to the mu-opioid receptor based on Surflex-Docking. Fully automatic flexible molecular docking (Surflex-Docking) was performed by using the possible active conformations of 70 fentanyl analogs and optimized 3D structure of mu-opioid receptor. The site mainly consist of residues ILE 109, ASP 112, TYR113, MET116, HIS262, TYR291. All these residues take part in interaction between fentanyl and mu-opioid receptor. Meanwhile, the results provide new insight to design of experiments aimed at understanding the structure.

Abstract: According to the electric field intensity of oblique twelve -needle electrodes (OTNE) in biological tissue, the discrete equations based on the indetermination linear current density were established by the boundary element integral equations (BEIE). The non-uniform distribution of the current flowing from twelve -needle electrodes to conductive biological tissues was imaged by solving a set of linear equations. Then, the electric field intensity generated by OTNE in biological tissues at any point can be determined through the boundary element method (BEM). It means that this method has an important referenced significance for computing the electric field intensity generated by OTNE in bio-tissue.

Abstract: It brings the pain to the patients whether in spirit or on physiology when laryngeal cartilage is injured. The paper reviewed the previous methods of improving the pronunciation for laryngeal cartilage injury and patients whose larynxes have been cut off, and compared the advantages and disadvantages of various methods. On the basis of the studies on the artificial auricle scaffold, artificial spinal cord scaffold in our center, this paper puts forward a larynx injury repair methods with a personalized tissue engineering artificial larynx, namely taking the advantages of biological manufacturing and tissue engineering related technology to prepare laryngeal cartilage scaffolds, and then the stem cells could be cultured into the scaffold in vitro. After more study and test, this bio-manufactured larynx scaffold could be expected as one of the ideal repair methods for the larynx injury.

Abstract: Six compounds were isolated from Gentiana farrerii, which was used as traditonal herb medicine in the treatment of some diseases. Their structures were identified as: linoleic acid(1), dibutyl-phthalate(2), p-hydroxy benzaldehyde (3), hydroxyacetophenone (4), isoscoparin (5), 5,7,3',4'-tetrahydroxyflavone-6--D-glucopyranoside(6). All compounds were isolated from Gentiana farrerii for the first time. The cytotoxic activity on the growth of HepG-2 cells of all compounds were investigated in MTT assay, the results showed that compound 2, 5, 6 have weak cytotoxic activity, IC50 is 41.26, 96.74, 99.33 (μg/ml), respectively.

Abstract: In the process of measuring human pulse rate, the motion of the measured object often make the pulse wave deformed and even distorted, which affect the precision of pulse rate seriously. To solve this problem, this paper proposed a new method for dynamical pulse wave calculation based on the comparison of slope summation. Collect pulse signals using GPTS(General Purposed Test Auto System), and search the peak with appropriate window width and step size . Adopting comparison of slope summation to identify the main peak of pulse, judging effectively and correcting the pulse rate within probability . Simulation and error analysis are proposed for statical and dynamical situation in this paper at last. The experimental results show that this method is generally applicable to dynamical detection , and the precision meets the national metrological verification procedures JJG 760-2003 .

Abstract: Medical images play a very important role in clinical medicine. They often have the features of low contrast, narrow gray scale and edge blurring between tissues. Aimed at the disadvantages, the paper presents a new medical image enhancement algorithm combining pseudocolor processing and marker-based watershed transform. The new algorithm and traditional pseudocolor processing in frequency domain are implemented using MATLAB, and their effectivity is evaluated by two parameters, mean and entropy. Results show that the proposed enhancement algorithm can improve the visual effect of different types of medical images, especially for the tissues in the low contrast regions in the image. The output image is more suitable for doctor’s diagnosing. The algorithm has been used in the medical image fusion system we developed, and the performance is very satisfactory.

Abstract: This paper analyzes the main management problems in drug circulation quality among pharmaceutical wholesale enterprises, and provides the construction strategy for a comprehensive quality safety control system of medicine circulation from a macro perspective. Based on the characteristics and function requirements of drug circulation management, it depicts structure model of the system, which includes three parts: sub-system of production management, perception management, and the regulatory decision-making. This paper mainly illustrates the design and research idea of the sub-system of sensor network and multi sensor management to meet the demand for the drug monitoring in circulation among pharmaceutical wholesale enterprises, that is early monitoring and warning in advance, rapid response and post evaluation and summary. The research results herein can offer beneficial reference in the further development for the system.