Advanced Materials Research Vol. 749

Abstract: This study investigated the cell apoptosis and gene expression profiles of human THP-1 monocytes in order to identify the molecular mechanism of cell apoptosis induced by meso-2,-3-dimercaptosuccinnic acid-coated Fe₃O₄ magnetic nanoparticles. Cell apoptosis was visualized with flow cytometry after treated by 50 and 100 μg/ml Fe₃O₄ nanoparticles, and the gene expression profiles were detected with Affymetrix Human Genome U133 Plus 2.0 GeneChips® microarrays. The transmission electron microscopy obserbation revealed that THP-1 cells were effectively labeled by the Fe₃O₄ nanoparticles. The internalized Fe₃O₄ nanoparticles increased cell apoptosis in a dose-dependent manner, but not decreased cell viability significantly. The cDNA microarray results showed that hundreds of genes were significantly regulated at the concentration of 50 and 100 μg/ml, and the level of these genes exhibited a dose response, including CD14, CD86, CFLAR, IL-1, NFKBIA, NLRC4, NAIP and AIP3. The Fe₃O₄ nanoparticles treatments resulted in significantly altered in Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, and Cell apoptosis signaling pathway. Gene ontology analysis of these differentially expressed genes demonstrated that mainly up-regulated genes were related to cytokine production and cell apoptosis. These results showed that the Fe₃O₄ nanoparticles induced THP-1 cells apoptosis and the level of lots of genes involved in extrinsic apoptosis pathway differentially expressed, which further revealed demonstrated the relation between Fe₃O₄ MNPs treatment and cell apoptosis.

Abstract: Objective:The aim of the article was to systematically evaluate a novel bone collector designed by authors for intraoral harvesting.Method: An electronic combined with a hand literature search was conducted and comparative analysis was performed focusing on the principle,quantity,decontamination and cost between author-designed collector and the one recommended in literature.Result: The collectors recommended in literature all are based on filter principle and contain different size of sieve for collecting bone debris which have the disadvantage of lower bone volumes,suction blocking easily and particularly susceptible to oral microbial contamination.While the bone collector designed by authors is based on dynamics principle and centrifugal separation principle and appeares to be higher efficient,effective decontamination in bone collection and low cost. Conclusion: The principle of the bone collector designed by authors was innovative and will have good application in ordinary native dental clinic for providing efficient osteogenesis,less trauma and less cost.

Abstract: Sodium alginate (SA)/polyvinyl alcohol (PVA) matrix-based wound dressing system containing ciprofloxacin hydrochloride, a topical anti-infective drug, were obtained by a casting/solvent evaporation method. The FTIR spectrum indicated that a strong interaction between SA and PVA and a good compatibility between ciprofloxacin hydrochloride and SA/PVA. The surface contact angles showed that the SA/PVA membrane has good compatibility. The water absorption and water retention rate increased when the concentration of PVA increased. The swelling ratio of SA/PVA mixtures indicated that the membrane go to balance in about 30min. Moreover, the release rate of ciprofloxacin hydrochloride was found to be increased with the increment of PVA content for all the composite samples. Thus, SA/PVA wound dressing systems containing ciprofloxacin hydrochloride could be a novel approach in wound care.

Abstract: Electrocardiography (ECG) is a diagnostic method that allows sensing and record the electric activity of heart [. The measurement of electrical activity is used as a standard twelve-point system. At each of these leads to measure the useful signal and interference was measured. The intensity of interference depends on the artefacts (electrical lines, brum, motion artefacts, muscle, interference from the environment, etc.). For correct evaluation of measured signal there is a need to processing the measured signal to suitable form. At present, the use of electrocardiograms with sensors with contact scanning are difficult to set a time so we decided to use the principle of non-contact sensing. Such a device to measure the ECG was constructed under the project. The disadvantage of such devices is a problem with a high level of noise, which degrades a useful signal. The aim of this article is to pre-process the signals obtained from non-contact sensing. The contactless devices are powered from the network and battery. The electrodes were connected by way of Eithoven bipolar leads. Signals were pre-treated with suitable filters so that they are also appropriate for their subsequent analysis. In the filtration ECG signals was used as a method of linear (low pass filter, high pass, IIR (Infinite Impulse Response) peak, notch filter. The results of many signals clearly demonstrate removing noise in the ECG signals to the point that is also suitable for their analysis.

Abstract: Once leafy vegetables are picked, metabolism and inoculating microbes affect the quality of the products badly. This paper included a brief introduction on the various factors affecting the freshness and shelf-life of leafy vegetables, with 3 types of treatments: antimicrobial gas or washing solutions (ozone, hydrogen peroxide, peroxyacetic acid, chlorine, electrolyzed oxidizing water, and silver solution), that are broadly applied on post-harvest leafy vegetables that help to reduce both spoilage microorganisms and pathogens.

Abstract: The present study was designed to investigate the effects of porcine collagen peptides on biological behavior of PC12 cells, the Amino acid composition of porcine collagen peptides was analyzed using Amino Acid Analyzer, the contact angles were measured with a goniometer, and after treatment with porcine collagen peptides, the impact of peptides on cells proliferation and differentiation was evaluated using MTT assay and Real-time PCR respectively. porcine collagen peptides is found to contain mainly Gly, Pro and Hyp, the contact angles of porcine collagen peptides surfaces were approximately 23.3°. MTT Results revealed that collagen peptides promoted PC12 cells proliferation at the given concentrations with its maximum effect at the concentration of 0.2 mg/ml (P<0.01), Real-time PCR Results revealed the poricine collagen peptides had no influence on the expression of specific neuronal marker β3-tubulin. Results of this study suggest that based on their bioactivity, porcine collagen peptides could be promising candidates for biomaterials.

Abstract: The plasmid (with resistant the herbicide glyphosate gene EPSPS and glufosinate resistance screening gene Bar) was transferred into soybean [Glycine max (L.) Merr.] by agrobacterium-mediated transformation, and the Bar gene as the selectable marker coupled with glufosinate as a selective agent is described. This method used for transformation requires without tissue culture steps. Soybean seeds were germinated for 1 day, plumule, cotyledonary node, and adjacent regions were wounded with the tip. The wounded half-seeds were inoculated with agrobacterium tumefaciens cells harboring a recombinant DNA that contained the EPSPS and Bar genes. Polymerase chain reaction and two herbicides screened proved that the EPSPS gene has been transferred into soybean successfully; the transformation rate of soybean about was 0.37%.

Abstract: In many previous studies, neuronal oscillations in humans under 1530 Hz activity have been observed, correlated with rhythmical motor output. In this present study, we observed the influence of non-rhythmical paroxysmal stimulation on cortical neurons by analyzing the coherence between electroencephalogram (EEG) and electromyogram (EMG). The non-rhythmical stimulation was performed by using this method that the braced force of the subjects one foot was released suddenly when the subject was standing still. The surface EMG signals from the tibialis anterior and the gastrocnemius muscles of 10 healthy subjects were recorded, and the cortical activities of contralateral cortical sensorimotor areas were also recorded simultaneously by EEG during the experiment. The data indicates that the range (10-25Hz) synchronization represents a state of the cortico-muscular network when the balance is broken which the braced force of subjects one foot is released suddenly.

Abstract: Parkinsons Disease (PD) is the second most common progressive neurodegenerative disorder and is referred as a leading cause of neurologic disability. The symptoms and signs of PD result from a decrease of dopamines level in the basal ganglia. Accordingly to this, exogenous substitution with dopamine agonists like levodopa, is used to correct the mechanical disorders at the early stages of the disease. Levodopa is referred as a standard in the treatment of PD. The modern studies of PD drug development and experimental therapeutics focuses on the concept of slowing and targeting the release of levodopa to prolong the therapeutic effect and reduce the number of administrations. The transdermal route was thought to be the best route for providing a progressive supply of levodopa to the systemic circulation. Alginate was chosen as a drug carrier because of its biocompatible and biodegradable properties and also because it has been widely used in drug delivery systems (DDS). The aim of this research work was to produce alginate membranes with and without levodopa. A solvent casting based methodology was used. Calcium chloride was assayed as crosslinking agent. Membranes were characterized using Differential Scanning Calorimetry (DSC) techniques. Drug release was evaluated using UV Spectrophotometry.

Abstract: deal scaffolds for tissue engineering should mimic the complex characteristics of natural tissues and their mechanical performance. This work presents a new concept of hybrid scaffolds produced through the combination of electrospinning and an additive bioextruder system. The obtained results have shown that the hybrid structures present improved mechanical properties