Key Engineering Materials
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Paper Title Page
Abstract: Bone cements incorporated with montmorillonite (MMT) were prepared in an attempt to
improve their mechanical properties. The cements were characterized using particle size analysis,
gel permeation chromatography, viscosity measurements, X-ray diffraction, transmission electron
microscopy, and mechanical properties. The average particle size and molecular weight of the
PMMA powders used were 47 μm and 100,000 g/mol, respectively. The incorporation of MMT led
to an increase in viscosity of the bone cement but did not severely affect its setting temperature or
the amount of residual monomer. Regardless of the MMT mixing methods used, in this case MMT
being mixing in liquid and powder components, sodium MMT (SMMT) was not well dispersed in
the bone cements, which was believed to be due to its hydrophilicity. Organophilic MMT (OMMT)
was better dispersed in the liquid component than in the powder component. The tensile and
compressive strengths of the bone cements with 0.5 wt% OMMT mixed in the liquid component
were 35.9 and 119.6 MPa, respectively, which were considerably higher than those of the bone
cements with 0.5 wt% OMMT mixed in the powder component (27.9 and 100.5 MPa, respectively).
753
Abstract: Thermoplastic polyurethanes are versatile polymers much used for biomedical
applications due to their mechanical properties and biocompatibility. Like most implantable
materials they are susceptible to bacterial colonization, particularly in applications at high risk of
bacterial contamination such as percutaneous catheters. The objective of this study was to assess
the antibacterial activity and the cell responses to a series of nanocomposite variants fabricated from
a polyether polyurethane and organically modified silicates containing either antibacterial
dispersing agents, non-antibacterial dispersing agents, or combinations of the two. The results
suggest that co-modification is a promising approach for modulating both bacterial and mammalian
cell responses to achieve appropriate antibacterial properties without cell inhibition.
757
Abstract: The effect of different hydroxyapatite particles on malignant melanoma cell was
evaluated in vitro. Two kinds of short-rode hydroxyapatite particles, which range from 0.4 to 1 um
and 0.5 to 1.5 um, were co-cultured with human’s malignant melanoma (MM) cell line A375 for 24,
48, and 72 h. Both of these HA particles showed the same cell proliferation rate as blank control,
and there was no statistically significant correlation of matrix metalloproteinases-2 (MMP-2)
expression to the HA particle size. SEM images showed that the membrane of tumor cell, which
co-cultured with HA particles, was shrunk. The cell superficial sentus was reduced and small size
particle’ effect was more obvious. Since immunohistochemistry and SEM data are qualitative
technique, further precise methods might bring more information about the effect of HA particles on
tumor behaviors. But this study can provide the bio-security test of micrometer HA particles in
transplantation after tumor excision.
761
Abstract: In vitro cell behaviors of calvarial osteoblasts (MC3T3) were evaluated by seeding them
on both the surface and inside of in situ hyaluronic acid-poly(ethylene oxide) (HA-PEO) hydrogel,
either after or before incorporation of mixture micro-particles of hydroxyapatite-β-tricalcium
phosphate on/inside the hydrogel, respectively. Cellular behaviors such as adhesion and
proliferation on the surface and inside the gel were evaluated with light microscopy and a
microplate reader by focusing on the interactions of cell-HA-PEO as well as cell-hydroxyapatitetricalcium
phosphate micro-particle surface in the gel. Cell adhesion and spreading seemed to be
enhanced by supplying the micro-particles to the inside the HA-PEO hydrogel, compared to the
results of the HA-PEO hydrogel itself.
765
Abstract: The achievement of biological sealing is determined by the quality of the skin attachment
on the surface of the percutaneous implant in the area where the implant penetrates the skin. It has
been known that certain surface features of the implants can significantly influence the interactions
between cells and substrate. In this study, titanium plates were bioactivated through
anode-oxidization firstly, and then cultured with human epithelium cells for 72h. Untreated Ti plates
were used as control. After the samples were dehydrated, the morphology of the cultured epithelium
cells was tested with Scanning electron microscopy (SEM). The surfaces of control group did not
enhance epithelium cell attachment and growth, while the bioactivated microporous surface of
anode-oxidized group would be beneficial to induce the formation of the pseudopod of epithelium
cell, and then interlock the human epithelium cells through the pseudopod, which imply that the
surface modification method of anode oxidization may be one of the most effective methods to
resolve the biological sealing.
769
Abstract: The purpose of this study was to investigate the effects of Galla Rhois, Psoralea
corylifolia, Camellia sinensis, Salvia miltiorrhiza and Platcodon grandiflorum on the inhibition of
artificial dental plaque formation and the cytotoxicity of the herbal extracts on human gingival
fibroblasts. The analysis was carried out with the measurment of dental plaque weight and the cell
survival rate of human gingival fibroblasts. It showed that Galla Rhois, Psoralea corylifolia,
Camellia sinensis and Salvia miltiorrhiza inhibited the artificial dental plaque formation and did not
reduce the cell viability of human gingival fibroblasts.
773
Abstract: We investigated phospholipid polymer hydrogels containing Fe3+ ions (PMA/PMB/Fe
hydrogel) for their use as antiadhesive materials in the healing tissues. These hydrogels were
prepared from the aqueous solutions of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-comethacrylic
acid) (PMA) and poly(MPC-co-n-butyl methacrylate) (PMB). The PMA/PMB hydrogel
is formed by the intermolecular interactions between PMA and PMB, and it reversibly dissociates
under physiological conditions. The addition of Fe3+ ions could control the gelation time and the
dissociation time. Mechanical properties such as the gelation time and viscoelastic properties can be
controlled by the FeCl3 concentration. With regard to biocompatibility, no evidence of
inflammation was observed in vivo. Therefore, the PMA/PMB/Fe hydrogel has a potential to be
used as an antiadhesive material.
777
Abstract: Stabilization of the fibrous protein collagen is important in biomedical applications. This
study investigated the efficacy of degradation control of collagen using (-)-epigallocatechin-3-Ogallate
(EGCG). EGCG treatment of collagen in solid state was carried out and collagen sponges
produced were characterized by measuring the physicochemical properties such as gel fraction, the
enzymatic degradability and cytocompatibility. According to gel fraction, EGCG-treated sponges
showed the increase of insolubility compared to intact sponges. It showed that EGCG played a role
in a crosslinker of collagen. Through in vitro enzymatic degradation test, EGCG-treated collagen
sponges showed significant enhancement of resistance to collagenase in comparison with 25 mM
EDC-treated collagen sponges. Also, cell proliferation assays showed that 40 mM EGCG-treated
collagen sponges exhibited similar cytocompatibility properties compared with tissue culture plate.
In summary, EGCG treatment of collagen sponges increased the stability of collagen. Therefore,
crosslinking of collagen based scaffold with EGCG imparted more desirable properties, making it
more applicable for use as a scaffold in tissue engineering applications.
781
Abstract: We investigated the bioconjugation of proteins on polymer nanoparticles covered with
bio-inert phosphorylcholine groups. Poly[2-methacryloyloxyethyl phosphorylcholine-co-n-butyl
methacrylate-co-p-nitrophenyloxycarbonyl polyethyleneglycol methacrylate] was used as an
emulsifier and a surface modifier to prepare the poly (L-lactic acid) nanoparticles by a solvent
evaporation technique. The diameter and surface potential of the nanoparticles were
approximately 260 nm and –5 mV, respectively. We considered that the polymer chain that
transformed the conformation by change in pH may be used as a method of controlling the bioreaction.
The enzyme activity in a different pH under the coexistence of poly (glutamic acid)
(PGA) and the enzyme was measured. Therefore, the enzyme activity increased in pH 6 in
PGA/enzyme mixture system compared with that in pH 7 while the activity was constant in the
enzyme single conjugation regardless of the pH change.
785
Abstract: Non-biofouling surfaces with polymer-based substrate were prepared for manufacturing
microfluidic devices. It was done by constructing biocompatible poly(2-methacryloyloxyethyl
phosphorylcholine(MPC)) brushes using surface-initiated graft polymerization method based on
dithiocarbamate as photoiniferter. The density and length of the polymer chains were varied by
changing the composition of the photoiniferter moiety in the base polymer (macrophotoiniferter)
and the photoirradiation time, respectively. The molecular weight and thickness of the poly(MPC)-
grafted chains were 320 kDa and 95±14 nm, respectively. Characterizations of the poly(MPC)
modified surfaces were conducted by water contact angle, X-ray photoelectron spectroscopy,
atomic force microscope. Protein adsorption resistance of these modified surfaces was then
investigated by contacting with human plasma protein dissolved in phosphate buffered saline. These
poly(MPC)-modified surfaces effectively reduced protein adsorption.
789