Applied Mechanics and Materials Vols. 675-677

Abstract: In this study, microwave-assisted extraction (MAE) was employed for the extraction of polysaccharides from Radix Astragali (PRA). The extraction parameters that influenced the extraction yield of PRA were optimized by orthogonal test design. The results showed that the optimal MAE conditions were as followings: liquid-solid ratio of 18 ml/g, irradiation time of 8 min, irradiation power of 300 W and extraction temperature of 65°C, while extraction yield of PRA was 8.39 %. The developed MAE method provided a good alternative for the extraction of PRA.

Abstract: Schisandra Chinensis Baill from four places were extracted with superficial CO₂ fluid extraction (SCDE) to explore the component differences based on regional. The condition of SCDE for Schisandra oil are: 18 MPa extraction pressure 6 Mpa , extraction temperature 40 °C and separate temperature for 90 minutes. The Schisandra oil were analyzed by high performance liquid chromatography (HPLC), using the oil yield and the lignin content as the quality index of the Schisandra. The results will be compared with the results of organic solvent extraction. For the extraction of Schisandra oil, SCDE is superior to the organic solvent extraction. The component differences of the Schisandra oil from four different places are obvious, and the oil yield and the lignin content of Schisandra from Changbai Mountain in Jilin Province is the highest.

Abstract: Sea buckthorn is a deciduous, hardy, multipurpose species, widely distributed all over the world. It contains different kinds of phytonutrients and bioactive substances, and which has a good potential for producing various processed products, especially its oil, desirable for nutraceuticals, medicinal and cosmetic purposes. The paper mainly reviews the nutritive and therapeutic values of sea buckthorn.

Abstract: This study was designed to evaluate the antioxidant activity of samples extracted from Jinhua ham. The activities of SOD and contents of MDA and NO were measured after mice were kept on feeding the four peaks separated by using chromatographic column. Peak-3 obviously enhanced the activity of SOD and MDA. Peak-1 and peak-2 had little influence on the activity of SOD and MDA. Peak-4 decreased their activities. All peaks obviously decreased content of NO. Results showed that these four peaks had excellent antioxidant activity. Finally, influences of sample extracted from ham on SOD activity, MDA content and GSH-PX activity in vitro of mouse liver and heart were also studied. Certain dose of samples would lead the decrease of SOD activity, MDA content and GSH-PX activity. This results showed that the sample have certain oxidant capacity.

Abstract: AIIM To explore the optimal ultrasonic extraction process of volatile oil from prunus tomentosa and analyze the component. METHODS The orthogonal test was carried out with volatile oil rate t as the index,the grinding degree of material, solvent, ratio of material to liquid, ultrasonic time as factors by ultrasonic extraction method,the components were analyzed by GC-MS. RESULTS The main factors that affect the extraction of volatile oil from Prunus tomentosa thunb is extraction, followed by ultrasonic time and.grinding degree. A₃B₂C₁D₃ was the best extraction condition: Selection of ethyl ether as extraction agent, the particle size is 30 mesh, the 1:10 ratio of material to liquid, ultrasonic time for 40 min. GC-MS techniques were applied to analyze the chemical constituents of volatile oil, six kinds of main components were isolated and identified ,accumulating for 92.82% of the total volatile oil content . CONCLUSION The Ultrasonic extraction time is short, easy operation, high yield, experiment verified that the extraction rate is 0.6270% under the optimum extraction conditions and identified six main components.

Abstract: This study was designed to evaluate the antioxidant activity of samples extracted from Jinhua ham by using such chemical assays as DPPH, scavenging hydroxyl free radical and ABTS. The results demonstrate that antioxidant capacity of the extracted sample is lower than oxidation capacity of Vc. The IC₅₀ spot of samples ranges between 0.6 % and 2.5 % (mass fraction of solute).

Abstract: The aim of study was to optimize the extraction process of ginsenosides and investigate the anti-fatigue effect of ginseng and acanthopanax extracts. Orthogonal test was used to optimize the extraction process, loading swimming experiment was used to observe the ant-fatigue effect, and BUN, LDH, CK, glycogen, T-SOD, MDA, GSH-Px and LD were taken as the anti-fatigue indeses to be observed. The yield of ginsenoside was 3.8%. The swimming time of mice in the treatment group was significantly prolonged compared with that in the control group (P< 0.05). The hepatic glycogen storage, LDH, GSH-PX and SOD in the treatment groups were obviously increased (P<0.05). Serum MDA and LD levels in the treatment groups were decreased, but no statistical significance compared with those in the control group. The serum BUN was significantly decreased in the middle-dose group (P<0.05). There was no significant difference in the serum CK between the treatment groups and the control group.LDH levels in the middle-dose and high-dose groups were significantly different from those in the control group. The ginseng and acanthopanax extracts can exert its anti-fatigue effect through increasing the amount of liver glycogen reserve and reducing the damage of negative metabolic products caused by an excessive exercise to the body.

Abstract: Liquid nitrogen cryotherapy is an effective treatment method for skin disease. However, due to the manual operation in the therapeutic process, the treatment effect is difficult to reach. Aimed this problem, the process of freezing in liquid nitrogen was explored. Firstly, the experiment of liquid nitrogen cryotherapy on pork with skin tissue is conducted to investigate the temperatures variation with time. Secondly, three-dimensional numerical simulation of liquid nitrogen cryotherapy was performed. According to the difference in the results of the simulation and experiment, the causes of differences are analyzed and related parameters are optimized. At last, the optimized parameters make the simulation results closer to the experimental results, and provide a reference for the simulation of human skin.

Abstract: BEL-7402, HT-29, SPC-A1, U-251 bioassay-guided fractionation led to the isolation of the three active constituents from a wild fungi, Boletus edulis Bull. Ex Fr., which collected from Changbai Montain area. These three compounds’ chemical structures were elucidated on the base of spectroscopic data and comparison with known compounds as 5-cholestene-2,3-oxide (1), β-sitosterol (2), stigmasterol (3). All of the three compounds were extracted from Boletus edulis for the first time, and compound 1 was significantly cytotoxic against the four human cancer cell lines (BEL-7402, HT-29, SPC-A1 and U-251) with IC50 values 6.29, 6.98, 4.79 and 7.54 μM, respectively. Results of the study could supply referencs to the people to find the new drug from the fungus.

Abstract: Objective To research the relationship between anti-tumor and of corn-cob sulfate polysaccharide in tumor-bearing mice .Methods Xanthosine oxidase and TBA colorimetryr methods were used to assay the contents of blood plasma SOD activities and MDA content. Results CCCS could increased the SOD activity (P<0.05) and decrease MDA (P<0.05) blood plasma of tumor – bearing mice .Conclusion The anti-tumor effect of CCCS may be due to enhanced anti-oxidative capacity in tumor-bearing mice.