Advanced Materials Research Vols. 1092-1093

Abstract: Carbonic anhydrase(CA)has a catalytic effect on calcium carbonate (CaCO₃) deposition, and can also control the deposition of CaCO₃. Screening strains that can produce carbonic anhydrase can solve the microbial CA from the natural environment in the role of CaCO₃ deposition. Through the single and orthogonal test, the present paper focused on temperature, pH value, culture time and inoculation of 4 factors to optimally culture the psychrotrophic strains, which could produce CA by independent screening from Huanglong of cold calcified water, in order to get high yield of CA psychrophilic strains. The results showed that the optimal condition of the 15-33 strains producing enzyme was temperature 15 °C, incubation time 12 h, pH 6.0, and inoculation 2.0 mL. The 18-10 strains^, was temperature 15 °C, incubation time 24 h, pH 6.5, and qinoculation 2.0 mL.

Abstract: Separation and purification of hypocrellin A (HA) and hypocrellin B (HB) are conducted for extracted concentrated solution of hypocrellin through dry packing of molecularly imprinted polymers (MIPs) of hypocrellin A and B and preparative solid phase extraction column. The result shows that the optimum packing column combination for mixed adsorption is H5 (i.e. MIP of hypocrellin A and B are respectively 10.5g and 4.5g), and that the elution sequence is hypocrellin A elution first and then hypocrellin B elution next after the eluent is changed.

Abstract: Through soil samples collecting and analysis in Hebei plain, we studied acidity characteristics and acidification sensitivity in Hebei plain. Results showed that the main reasons for decline in soil pH value is the atmospheric acid deposition, the excessive application of nitrogen and phosphorus fertilizer, crop base ion absorption. The dissolve of calcium carbonate is the main process plain cushion soil acidification in Hebei plain, its content is the decisive factor of soil acidification sensitivity.

Abstract: D301 resin was used to separate and purify total anthraquinones in Radix et Rhizoma Rhei. It was found that the optimized dynamic adsorption conditions is pH7.0, flow velocity of 1.0 BV/h and extracting solution with concentration of 0.3g/mL and optimized dynamic elution conditions is hydrochloric acid with concentration of 0.15mol/L, 75% ethanol, and flow velocity of 1.0 BV/h. Under the above conditions,the average adsorption rate and desorption rate are respectively 89.75% and 91.15%, indicating that extraction efficiency of total anthracenone of emodin is relatively high and that the technology enjoys strong stability.

Abstract: In order to more deeply understand the mechanism between microorganism and atmospheric inhaled mineral fine particles. Albite, the typical mineral fine particle in the atmosphere, was used as the research object. This study investigated the air common bacteria-Staphylococcus aureus acted on albite for 7 days. By inductively coupled plasma (ICP), zeta potentials analyzer, fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) testing means, we can analyse soluble ions, surface charge, surface groups and surface morphology of albite. The results were listed as followes: 1 Staphylococcus aureus could promote the dissolution of albite, and promote the soluble Si, Al and Fe ions; 2 Staphylococcus aureus would adsorb on albite surface, gave its some characteristics to albite, changed surface charge, and made the zeta potential move in the positive direction. The above results are important for the establishment of the inhaled fine particles of mineral environmental and health impact assessment system.

Abstract: In this paper, the salinity domestication and mechanism of nitrite nitrogen removal ofAcinetobacter lwoffii were studied. The results showed that the optimum domestication salinityof the bacteria was 0.5% NaCl. It was speculated that the bacteria might have both oxidase andreductase, transform the nitrite nitrogen into ammonium nitrogen and nitrate nitrogen, and then usethem to synthesis the body protein. It also found that high concentrations of sodium nitrite hadinhibition effect on the bacteria growth.

Abstract: Study on effects of climate change on species is very popular and necessary at present. This paper investigated the effects of climate change on the distribution of snub-nosed monkey in China using Arcgis and Maxent model. It compared the historical and modern distribution of snub-nosed monkey in China, and evaluated the effects of climate change on Rhinopithecus bieti to explore effects on the distribution of snub-nosed monkey, so as to protect them. Results indicate that the distribution range of snub-nosed monkey has decreased greatly from the previous 17 to 8 provinces. The distribution trends are from north to south, east to west, dense to sparse and form several isolated distribution areas. Moreover, six precipitation variables are identified as having remarkable effects on the habitat suitability of Rhinopithecus bieti and therefore protective actions can be taken accordingly.

Abstract: This paper compares the inhibitory effect of high CaCl₂ and MgCl₂ content on anaerobic microorganism based on kinetics model. Salt inhibition kinetics experiments were performed at granular sludge and activated sludge in order to investigate salt inhibition effects on kinetic constants. Half-velocity constant values considerably increased when CaCl₂ or MgCl₂ concentration increased from 0 to 35g/L for granular sludge and activated sludge. Salt inhibition biokinetic coefficients (K_ρ) were obtained by using Ghose and Tyagi model. For granular sludge, K_ρ of CaCl₂ was found to be 130.3 g/L, and K_ρ of MgCl₂ was found to be 86 g/L. For activated sludge, K_ρ of CaCl₂ was found to be 92.8 g/L, and K_ρ of MgCl₂ was found to be 77.6 g/L. Results showed that the inhibitory CaCl₂ limit was higher than the inhibitory MgCl₂ limit for anaerobic microorganisms.

Abstract: The phytoplankton community were investigated at Xingkai Lake in July and August 2011. The results showed that there were 31 species belonging to 5 phylum of phytoplankton in Xingkai Lake. In the detect, 10 species (32.29%) of Cholorophyta was found. Cell density in Dangbi town and Lake center was 1.592×106ind./L and 1.562×106ind./L respectively. Cell density of Cyanophyta was the highest, and the dominant species were Merismopedia tenuissim, Anabaena sphaerica and Phormidium anomala . Shannon-Wiener index of samples in the two places were 2.85 and 2.86 respectively, and Margalef index were 1.59 and 1.27 respectively. It showed that the type of water was mesosaprobic-heavy pollution type, and we should pay attention to the water pollution.

Abstract: A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of hypocrellin A, hypocrellin B from Fermentation Broth of Shiraia bambusicola LPHP-89. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase consisted of methanol、water and acetic acid (60:40:1, v/v/v) and was pumped at a flow rate of 1.0 mL/min. The injection volume was5 μL. The quantitation wavelength was set at 254 nm.Total run time was 20 min and retention times for hypocrellin A and hypocrellin B were 10.06 and 15.38 min, respectively.