Abstract: Niobo-phosphate glasses were produced in order to assess the citotoxicity of samples
with different compositions. Different P2O5/Ca ratios were studied in an attempt to correlate the
biological behavior of new niobo-phosphate bioactive glasses with conventional bioactive calcium
phosphate glasses. The most biocompatible glass composition was chosen to produce glassreinforced
Abstract: Glass-ceramic scaffolds for bone grafting have been prepared using the sponge
replication method and a highly bioactive silica based glass belonging to the system SiO2-P2O5-
CaO-MgO-Na2O-K2O (CEL2). The parameters for the sponge impregnation were optimized in
order to obtain a continuous coating of the polymeric skeleton which will lead, after the polymeric
phase removal, to a resistant macroporous glass-ceramic template. A complete characterization was
carried out on the obtained scaffold in order to assess its morphology and specifically its porosity
and the degree of pores interconnection. A human osteoblasts cells line (MG-63) was cultured onto
the scaffold and the effect of stimulation with shock waves on the cell ability of colonizing the
scaffold was investigated.
Abstract: In this work a new kind of CaSiO3-doped α-Ca3(PO4)2 ceramic materials, with
compositions lying outside the field of the Ca3(PO4)2 solid solution in the system Ca3(PO4)2-
CaSiO3, were obtained and some of their properties, relevant for bone repairing, were studied in
vitro. Crystalline α-Ca3(PO4)2 solid solution and minor amounts of non-equilibrium residual glass
were the only phases in the materials containing 2 and 5 wt% of CaSiO3. α-Ca3(PO4)2, crystalline
eutectic-like phase and residual glass were observed for sample containing 15 and 20 wt% of
CaSiO3. The mechanical strength improved for all the doped ceramics with regard to un-doped
Ca3(PO4)2. The release of ionic Ca and Si in simulated physiological conditions increased with the
content of CaSiO3 and favored α-Ca3(PO4)2 surface transformation. The soluble components
extracted from the CaSiO3-doped α-Ca3(PO4)2 bioceramics were not cytotoxic to human fibroblastlike
cells. Initial cell adhesion onto the surface of the materials seemed to be partially hindered by
surface reactivity and remodeling, however those cells adhered to the experimental bioceramics
were viable and proliferated normally.
Abstract: In this work, a new bioresorbable phosphate glass (I-CEL2) was prepared in order to use
it for the production of 3D-bioresorbable scaffold for bone regeneration. I-CEL2 was characterized
to assess its thermal characteristics as well as its bioresorption rate in different medium such as
distilled water, Tris-HCl and Simulated Body Fluid (SBF). 3D-macroporous scaffolds were
prepared by mixing and pressing I-CEL2 powders and an organic phase and by treating the compact
of powders at 550°C for 3 hours. The obtained scaffolds showed a very high porosity and a high
resorption rate and are thus suitable candidates for a scaffold to be used as a temporary guide for
bone regeneration. The initial response of human marrow stromal cells (hMSCs) has been tested on
I-CEL2 surface to describe its biological potential.
Abstract: Bioglass films were prepared by reactive magnetron sputtering from a 45S5 target and than
annealed in air up to 550 oC in order to relax the mechanical stresses. The mixtures (0, 7 and 15%) of oxygen
and argon were used in order to modify the films stoichiometry. Morphological and compositional studies
were performed by SEM coupled with an EDAX system. FTIR and micro Raman measurements were
performed in order to investigate the molecular vibrations of the BG structure. A modified composition
respect to the target one was found for all the films, with lower silicon dioxide concentrations. The films
displayed rough surfaces with spherical particles of 10-20 μm diameter embedded in the surfaces. The
Raman results evidenced important carbonate lines besides the silica-phosphate bands.
Abstract: Apatite forming and bonding ability of Ti based bulk metallic glass
((Ti0.40Zr0.10Cu0.36Pd0.14)99Ca1: BMG) were investigated as a new type of biomaterials. Powder
mixture of CaHPO4･2H2O and Ca(OH)2 and the BMG disks were treated with autoclave for
hydrothermal hot-pressing simultaneously (150°C, 40MPa). And then the BMG disks were soaked
in simulated body fluid (SBF, Kokubo solution). Apatite were deposited and covered on the
surface of the BMG. It was firstly demonstrated that surface of Ti based bulk metallic glass could
be revealed apatite forming ability.
Abstract: The RF magnetron sputter technique was used to deposit Bioglass (BG) and
hydroxyapatite (HA) coatings onto titanium substrates. The aim of this study was evaluated
the growth behavior of rat bone marrow cells of various deposited coatings.
The EDS measurements demonstrated that the composition BG coating was changed during
magnetron sputtering. The rat bone marrow derived osteoblast-like cells showed improved
osteogenic response on crystalline magnetron sputtered HA coatings compared BG coatings.
Scanning electron microscopical examination showed an extensive mineralization after 16
days of culture, while on the surface of the BG coating only a multilayer without
mineralization could be observed.
Abstract: The results of a combined structural characterisation (XRD, IR, NMR, SEM, TEM) of a
phosphate containing Mg-Ca silicate and a phosphate containing Na-Ca silicate glass samples are
presented. The structural results are also compared with in vitro tests carried out in simulated body
fluids for checking bioactivity.
Abstract: The aim of this study is to investigate the crystallization behavior and in-vitro bioactivity
character of glass ceramics having 3:7 weight ratio of fluorapatite (Ca10(PO4)6F2) to potassium mica
(K2Mg3AlSi3O10F2) as a function of cerium oxide addition, and compare the morphology of
hydroxycarbonate apatite (HCA) layer formation depending on cerium oxide addition.
Abstract: In this study, we report on the effect of Bioglass® structural transformations on its
sintering behaviour. In a previous paper, we showed that while heating up to 1000°C, five
successive transformations occur: glassy transition, phase separation, two crystallization
processes and a second glassy transition. The sintering of the material exhibits two main
shrinkage stages associated to the two glassy transitions at 550°C and 850°C. At 580°C, the
glass-in-glass phase separation induces a decrease of the sintering rate immediately followed
by the crystallisation of the major phase Na2CaSi2O6 between 600 and 700°C, from the
surface to the bulk of the particles. A completed inhibition of sintering takes place followed
by a minor shrinkage due to volume crystallization. A plateau is observed until the second