Applied Mechanics and Materials Vols. 138-139

Abstract: A convenient method to synthesize disodium salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) phenyl phosphate (OXDP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via an one-pot procedure of condensation between the benzoic acid and 2-hydroxybenzhydrazide at triethylamine alkaline solution in the presence of propylphosphonic anhydride, was realized. Preliminary tests reveal that the lessened fluorescence intensity resulting from enzyme reaction is proportional to ALP activity in the range of 1.0-25.0 U/L with the detection limit of 0.25U/L, which can be successfully applied to assess ALP in human serum with good precision.

Abstract: In this paper, a new method based on Supervised Kohonen network (SKN) and Mel-frequency cepstrum coefficients (MFCC) is introduced. MFCC of heart sound signal are extracted firstly, and then features are got by calculating every order of MFCC average energy. Finally, SKN is used to identify heart sound. The experimental result shows that this algorithm has a good performance in heart sound clustering, and is of significant practical value.

Abstract: The preparation and humidity sensing of nanorod-like ZnO materials are studied. Firstly, the ZnO nanoparticles with high specific surface area are prepared via chemical solution growth techniques. Then, some ZnO nanoparticles are manipulated by dielectrophoresis in interdigital electrodes to assemble a capacitive-type humidity sensor. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) are used to determine the structural and morphological properties. The results show that the sensor prototypes have high sensitivity, and it is the potential sensing material for high performance humidity sensors.

Abstract: A novel nickel nanocomposite electrode supported by 3D ordered silicon microchannel plates (MCP) had been reported and its electrocatalytic toward the oxidation of glucose for sensor had been studied. The 3D ordered Si MCP electrodes were first fabricated by electrochemical etching and then Nickel nanoparticles were deposited onto the sidewall of the MCP via electroless deposition followed by annealing at 300°C for 300 s under argon to stabilize the structure. The morphology of the Ni/Si-MCP electrode was characterized by Scanning electron microscope (SEM) and X-ray diffraction (XRD). The electrochemical methods were employed to investigate the Ni/Si-MCP materials. The Ni/Si-MCP nanocomposites exhibit superior electrocatalytic properties towards glucose electro-oxidation in alkaline solutions, in addition to showing excellent long-term stability and good reproducibility.

Abstract: GH425 gene comes from the Grimmia pilifera drought stress of cDNA library.In this experiment,we have got the full sequence of GH425NO.1 by E-cloning which using GH425 as gene probe,in Physcomitrella patens DNA Datebase.Through using ORFfinder to find out the longest ORF and design primer for it,then, validated the Physcomitrella patens by PT-PCR,and we have obtained corresponding band and proved that the result of silicon cloning is correct and the fragment is contained in Grimmia pilifera P.Beauv.Now,we know the sequence encodes Eukaryotic initiation factor 4E by Blastx,and analysis it with Bioinformatics.

Abstract: Juglone (1), plumbagin (2) and isoplumbagin (3) were synthesized from the natural product 4,8-dihydroxy naphthol-β-D-glucoside (4) which existed large amount in barks of Juglans mandshurica. Juglone was achieved directly by deglycosylation catalyzing with CAS in 78 % yield. Plumbagin was synthesized by five steps with an overall yield of 22 %. Isoplumbagin was synthesized by six steps in 24 % yield.

Abstract: Fermentative hydrolysate of extruded corn gluten with higher solubility and antioxidative property by Bacillus natto was prepared. Extrusion of corn gluten was applied as pretreatment before fermentation. The best fermentative hydrolysis results can be obtained by fermenting at 34°C for cultivation time of 32 h, and flask rotation speed and initial pH of culture media were 200 r/min and 6.5-7.0 respectively. Soluble protein content and antioxidative activity of the fermentative hydrolysate reached 24.95 mg/ml and 259.21 U/ml respectively under the optimized culture condition. The molecular weight of the peptides in the optimal hydrolysate distributed mainly over 860-5300 Da determined by gel filtration chromatography. The hydrolysates displayed good solubility and antioxidative activity.

Abstract: This paper reports the concentration of heavy metals (Cr, Cu, Zn, Cd and Pb) in the soils and rices surrounding the abandoned rural waste dumping sites in Ningbo. Igeo (geoaccumulation index) was calculated to assess the contamination degree of heavy metals in soils. The mean contents of Cr, Cu, Cd, Zn and Pb of soils were 33.3, 24.1, 1.5, 118.9 and 45.6 mg/(kg DW) (dry weight), respectively. All of them were much higher than that of the reference value (i.e. CK), but there were no coherent trend of the metal contents within 1-120m distance from the dumping site. Igeo of heavy metals reveals the order of Cd>Cu>Cr>Pb>Zn, and the contamination assessment of soils using Igeo indicate the moderate Cd pollution, while the soils were unpolluted-moderately overall by Cr, Cu, Zn as well as Pb. The heavy metal contents in root, stem & leaf and rice grains were all remarkable higher than that of the CK at 20-120 m distances, and the heavy metal contents in root were evidently much higher than other plant parts, while those in rice grain were lowest, indicating the great bioaccumulation trend of heavy metals. Although the metal contents in the rice grain were within the legislation limit, its bioaccumulation trend of heavy metals was remarkable, whose contents were 4.38-fold for Cr, 1.76-fold for Cu, 1.28-fold for Zn, 2.67-fold for Cd and 3.03-fold for Pb higher than that of reference value, respectively. Finally, we proposed a decentralized in-situ restoration approach for the dumping sites.

Abstract: To investigate the extraction technique of soluble soybean polysaccharide by ultrasonic assistance, four relative factors, including water extraction temperature, ultrasonic treatment time, ultrasonic power and solid to liquid ratio, were optimized through orthogonal test L9 (34 ) on the basis of single factor experiment. The factors influencing the extraction rate of soluble soybean polysaccharide, in the sequence of significance, were ultrasonic treatment time, water extraction time, water extraction temperature and sold to liquid ratio. The optimum extraction technological parameter of soluble soybean polysaccharide was: ultrasonic wave period 20 min, water extraction temperature 90 °C, sold to liquid ratio 1:40, water extraction period 4 h, and the extracting rate was 3.5421% of bean dregs. The method of phenol-sulphate acid was applied to determine the content of polysaccharide. The content of polysaccharide was 96.58％ of extracted soybean polysaccharide sample.

Abstract: The partition behavior of Puerarin in ethylene oxide-propylene oxide random copolymer (EOPO)/ K₂HPO₄/water systems was investigated .The optimum conditions were EOPO （SDP30） 28%, K₂HPO₄ 24%, temperature-induced condition was 70°C and 30min. yield of Puerarin could reach 42.26%. Based on the modified model lnK/R2=A*+b*(w”-w’)+c*(w”-w’)2, partition coefficients of Puerarin in EOPO/K₂HPO₄ aqueous two-phase systems were correlated. It was found that the correlation of the experimental data was quite satisfactory.