Key Engineering Materials
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Key Engineering Materials Vols. 342-343
Paper Title Page
Abstract: This study evaluated periodontal repair and biomaterial reaction following implantation of
a newly fabricated calcium phosphate chitosan block bone and chitosan membrane on the
regeneration of 1- wall intrabony defects in the beagle dogs. The surgical control group received a flap
operation only, while experimental group was treated with calcium phosphate chitosan block bone
and/or chitosan membrane. All dogs were uneventful healing without any complication. The subjects
were sacrificed 8 weeks after the experimental surgery, and a comparative histological and
histometric examination was done. Chitosan membrane group significantly enhanced bone
regeneration compared to control, CaP-chitosan block bone and combination group. Chitosan
membrane remnants were observed in chitosan and combination group, while CaP-chitosan bone
materials were resorbed completely. These results suggest that Chitosan membrnae significantly
enhanced bone and cementum formation in advanced periodontal defects.
393
Abstract: Thermosensitive scaffold was suggested for the partial replacement of nucleus in
degenerated intervertebral disc with a minimally invasive surgical procedure. Biocompatible and
thermosensitive scaffolds were prepared by coupling reaction of Pluronic with chitosan and it was
inserted in degenerative spinal motion segments. To confirm the regeneration of degenerative discs
and subsequent structural stability, cell proliferation and morphological changes were evaluated
using relaxation time, quantity of DNA and histological examination. As a result, inserted group
showed higher relaxation time, reduced the decrement of DNA contents, and accumulated GAG
amount. The results confirmed the potential of thermosensitive scaffolds and minimally invasive
surgical procedure for the regeneration of degenerative discs.
397
Abstract: Glucans have been reported to stimulate immunity and to promote wound healing. Adult
human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF
was measured at various concentrations of β-glucan by MTT assay, and migration was observed for
36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study,
the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were
examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF
migration speed, and has the potential as a wound healing agent.
401
Abstract: Mechano-active scaffolds were fabricated from very elastic
poly(L-lactide-co-ε-carprolactone). The scaffolds with 80 % porosity and 300~500 μm pore size
were prepared by a gel-pressing method. As a control group for elastic properties of polymer
scaffolds, rigid poly L-lactide scaffolds were fabricated. The scaffolds were seeded with
chondrocytes and cultured to evaluate the effect of elastic properties of polymer scaffolds for the
differentiation and the ECM secretion of chondrocytes. Also, the chondrocytes-seeded constructs
were implanted in nude mice subcutaneously to investigate their biocompatibility and cartilage
formation. From the biochemical analyses, chondrogenic differentiation was sustained and
enhanced significantly and chondral extracellular matrix was increased through mechanical
stimulation of dynamic environment in the dynamic body systems. Histological analysis showed
that implants of PLCL constructs formed mature and well-developed cartilaginous tissue, as
evidenced by chondrocytes within lacunae. Consequently, the elastic PLCL scaffolds could be used
to engineer cartilage in mechanically dynamic environments
405
Abstract: Mechano-active scaffolds were fabricated from very elastic
poly(lactide-co-carprolactone) by a gel-pressing method. The scaffolds were seeded with bone
marrow stromal cells and the continuous compressive deformation was applied to cell-polymer
constructs in the chondrogenic media. Then, they were implanted in nude mice subcutaneously to
evaluate for the effect of dynamic compression for regeneration of cartilage. From the biochemical
analyses, chondrogenic differentiation was sustained and enhanced significantly and chondral
extracellular matrix was increased through mechanical stimulation. Histological analyses showed
that implants stimulated mechanically formed mature and well-developed cartilaginous tissue, as
evidenced by bone marrow derived chondrocytes within lacunae. Consequently, the periodic
application of dynamic compression can encourage bone marrow stromal cells to differentiation to
chondrogenic lineage and to maintain their phenotypes.
409
Abstract: The goal of this study was to investigate release tendency of brain-derived neurotrophic
factor (BDNF) from poly(L-lactide-co-glycolide) (PLGA) and small intestine submucosa (SIS)
scaffold prepared by ice-leaching method. A porous scaffold consisting of PLGA and SIS as carrier
of BDNF has been prepared in the presence of ice particle. SEM image of the PLGA/SIS scaffold
showed an interconnected pore structure. The release behavior of BDNF loaded PLGA/SIS scaffold
was examined for 4 weeks period at phosphate buffered saline (PBS, pH 7.4) at 37 oC. The
sustained release of BDNF over 4 weeks was observed from the PLGA/SIS scaffold. These results
indicate that the sustained release of BDNF from PLGA/SIS scaffold can be very useful for
application in the tissue engineering.
413
Abstract: Microcapsules of alginate cross-linked with divalent cations are the most common
system for cell immobilization. In this work, the polyion complex (PIC) microcapsules were made
using sodium alginate/barium chloride as the wall materials and gelatin/poly (vinyl alcohol) (PVA)
as the extracellular matrices. The result of the permeability experiment of microcapsules using
proteins with different molecular weight showed that the capsule has a molecular weight cut-off
(MWCO) of 150 kDa. The hepatocytes encapsulated in microcapsules with gelatin and PVA in the
core rapidly aggregated as incubation time increased. The aggregated hepatocytes showed high
ammonia removal and albumin synthesis, showing a high potential for use in a bio artificial liver
system.
417
Abstract: Hydrotropic block copolymers, consisting of a hydrophilic poly(ethylene glycol) (PEG)
block and a hydrotropic polymer, poly(2-(4-(vinyl benzyloxy)-N,N-diethylnicotinamide))
[P(VBODENA)], block, were synthesized by atom transfer radical polymerization (ATRP) for
aqueous solubilization of paclitaxel, a representative poorly water-soluble drug. These polymers
showed an excellent solubilizing effect for paclitaxel in aqueous media in comparison with the
corresponding hydrotropic agent and a control micelle (PEG-PLA) and such effect was significantly
dependent on the polymer concentration and composition. Paclitaxel could be solubilized into
polymer micelles in aqueous media without use of an organic solvent. Due to their promising
properties such as micellar characteristics and hydrotropic solubilization, the hydrotropic polymer
micelle system can be useful for formulation of paclitaxel and other poorly soluble drugs.
421
Abstract: Vectors are vital aspect of gene delivery system which decides the success of gene
therapy. Efficient transfection with minimum or no toxicity, are two principal aims of any gene
delivery system. In this our study, we rationally developed biodegradable water soluble poly(ßamino
ester) (PAE) based on spermine (SPR) and poly (ethylene glycol) (PEG), by Michael-type
addition reaction and further studied for its potential as a gene carrier. Confirmation of synthesized
PAE was done by proton NMR spectroscopy. In gel retardation assay, the PAEs have shown good
DNA binding ability over wide range of polyplexes. The addition of PEG over SPR resulted in a
novel PAE with higher degree of safety and transfection efficiency as compared with
polyethylenimine 25K (PEI) when studied in 293T human kidney carcinoma cells.
425
Abstract: The objective of this study is to develop a new type of cationic nanoparticles for the
intracellular drug delivery to breast cancer. Poly(ester amine) (PEA) based on polyethylenimine and
polycaprolactone was synthesized to make cationic PEA nanoparticles for all-trans retinoic acid
(RA). In the 1H-NMR study, the proton signals of RA appeared in the spectrum of RA-loaded PEA
nanoparticles in CDCL3, whereas they disappeared in D2O, suggesting that hydrophobic inner-core
with hydrophilic outer-shell formed in water. RA release was faster at lower drug content and RA
was released over a period of 20 days. RA-loaded PEA nanoparticles showed enhanced cytotoxicity
compared with RA itself, whereas nanoparticles of PEA themselves did not show it. These results
indicated that the cationic PEA provided an efficient intracellular delivery of RA.
429