Advanced Materials Research Vols. 524-527

Abstract: Green agriculture is an inevitable choice for the development of agriculture and is a main mode of agricultural production to maintain sustainable. Assess the function of green-agriculture ecosystem services is the basis to establish the ecological compensation mechanism and the standard of compensation. This paper analyzes the feasibility that CVM’S application in Yingshan green agriculture non-market valuation through sorting the CVM’S application status.

Abstract: The potential of aqueous two phase extraction for the isolation and purification of flavonoids from Artemisia argyi leaves is investigated. Influence of the process parameters such as type of phase forming salts, concentration of salt and ethanol, added salt and temperature was studied. 30% ethanol/18.87% (NH4)2SO4 system was found most suitable for the extraction. The ethanol and salt concentration were found to significantly affect the flavonoids recovery and partition coefficients. The recovery of 93.16% was obtained at the selected process conditions. The results demonstrated the feasibility of aqueous two phase extraction for the isolation and purification of flavonoids.

Abstract: To learn about banana microstructure changes on affecting moisture dehydration performance and its quality during oven drying process, this experiment chose scanning electron microscopy to observe the microstructure changes on outer surface, inner surface and cross section of banana peel and its flesh during drying process, in order to examine the relationship between dehydration performance and its microstructure shape and microstructure pore structures changes. The results showed that during drying process, all surfaces of banana peel and its flesh endured shape deformation from regularly to irregularly. Microstructure shrank evenly in beginning, while became conglomerate later, appeared to be significantly distortion and breakage. Microstructure pore quantity and area showed great changes, those average diameter over 10um pore numbers increased steadily, while pore area just reduced in beginning and then increased rapidly afterwards. As banana microstructure shape and pore shrinkage and deformation increased greatly, which also increased the resistance of moisture evaporating greatly. This may be an important reason that cause banana drying speed and quality decreased, it is an urgent problem that needs to be solved during fruit and vegetable drying process and storage.

Abstract: Sour pickled cabbage is a traditional Chinese food, which was made with fresh vegetables such as cabbage. Because of its naturally fermentation, a lot of lactic acid bacteria are riched in it. However, the traditional naturally fermented sour pickled cabbage were home-made in China, because of the difference of raw material, processing, formula and other factors, the flavor, nutrition and safety of them varied significantly, in order to investigate the influencing factors affected the flavor, quality and security of the naturally fermented sour pickled cabbage, and discuss the relationship between chemical composition and the flavor, quality and security, the acidity, total sugar, nitrite, protein and sodium chloride levels of 5 naturally fermented sour pickled vegetables by means of acid-base titration method, the direct titration, the hydrochloric acid naphthalene ethylenediamine kieldahl method, the indirect precipitation titration method respectively. The results were showed as follow, the acidity were between 0.283% and 0.891%, the total sugar content were between 3.96% to 4.37%, the nitrate content were 0.167 g/kg to 0.267 g/kg, the protein content were between 0.0169% and 0.0218%, the sodium chloride content were between 0.412% and 0.447% respectively.

Abstract: The chemical composition of the essential oil of Mentha spicata from China was investigated by the Gas chromatography/mass spectrometry (GC/MS) analysis. 21 compounds were identified, representing 97.10% of the total oil. The major compounds in the essential oil were carvone (65.33%), limonene (18.19%), dihydrocarvone (2.97%) and camphene (2.34%). The oil showed antioxidant activity assessed using DPPH assay. The antimicrobial activity was investigated in order to evaluate its efficacy against 5 microorganisms using MIC and MBC methods. The results indicated that spearmint oil showed inhibitory and bactericidal effect on all test microorganisms. It was highly effective against E. coli, S. cerivisiae and P. citrinum. S. cerivisiae was more susceptible to spearmint oil (MIC 0.78125 uL/mL and MBC 6.25 uL/mL). The cytotoxicity of spearmint oil was evaluated on Hela cells using the MTT assay. The oil showed quite strong cytotoxicity on the cell lines with an IC₅₀ of about 2.08ug/mL.

Abstract: Combine thin layer chromatography ( TLC ) with high performance liquid chromatography ( HPLC ) detection of kudzu root isoflavones, to establish a rapid and accurate method to determine the component content of Puerarin and daidzin in Radix Puerariae.Though study on the optimization analysis of Puerarin Content in Radix Puerariae and daidzin content chromatography in a variety of conditions, including TLC developing solvent and options of coloration, and HPLC chromatographic separation conditions and detection characteristics of TLC and HPL, to get the best Chromatographic separation conditions and make a comparison with Puerarin pharmacopoeia test methods.The experimental results show that: TLC has the advantages of simple operation, rapid, reproducible, rapid identification of Puerarin and daidzin , HPLC with good stability, great accuracy and high precision that can be detected in puerarin and daidzin content. TLC and HPLC is a simple, fast, accurate method and contribute to the establishment of puerarin medicine standardized quality evaluation system and comprehensive utilization and development of Pueraria resources.

Abstract: This research focuses on studying the effects of biochar on acidic soil nutrient, soybean production, root and plant height,using the method of pot plant simulation for soybean cultivation patterns in three ridging test. Biochar was applied for pot with set 0, 1, 5, 10, 15, 20, 25t hm-2. Biochar, diammonium hydrogen phosphate, urea and potassium chloride were fully mixed then sampled on soybean clover stage (V3), full bloom stage (R2) and the early mature stage (R7).This research examined the soybean root index. At the early mature stage (R7), soil samples were blown dry and examined chemical index. During the mature period (R8), we examined the yield and plant height. The results showed that, with the increase of the content of the biochar, available potassium，and organic matter was increased significantly, increasing 77.69mgkg-1, and 7.56gkg-1 compared with control respectively. The root dry weight, main root length, lateral root number, total length, lateral root number, root dry weight of the maximum was respectively increased by 80.85%, 100%, 33.86%, 167.74%, 26.31% and 80.41% than control in early mature stage. The yield increased by 35.97% compared with control.

Abstract: Lactoferrin has many kinds of bioactivities which have attracted more and more attention. In the present study, lactoferrin from bovine milk was isolated and purified by membrane filtration, series of chromatography on SP Sepharose Big Bead ion exchange column and Superdex 200 gel filtration column. The purified lactoferrin was identified by SDS-PAGE compared with the lactoferrin standard.

Abstract: Purification extraction of ferulic acid from wheat bran by using ultrafiltration,based on the molecular weight cutoff (MWCO) of ultrafiltration membranes,ultrafiltration pressure,sample concentration,ultrafiltration time these four factors influence of the membrane flux,determine the best the ultrafiltration conditions.

Abstract: Grain is a vital commodity that has significant effects on people’s livelihood. Grain is not an infinite renewable resource due to population growth, shrinking cultivated land, limited water supply, ongoing climate change, and so on. In spite of multiple varieties, grain faces serious challenges now and in the coming decades in china. Where there is short of individual grain breed suitable for end-use performance. The farrago of different varieties leads to inefficient use of grain. Improved detection methods are so scarce that grain is not graded in the light of end-use quality. NIR provides a more rapid, objective, and reliable means for the quality evaluation of grain. The paper describing the practical applications of NIR in the entire grains value chain clarifies a viewpoint of improving the utilization efficiency of grain resources by NIR in China.