Advanced Materials Research Vols. 183-185

Abstract: Bifidobacterium bifidum were encapsulated as fresh cultures in water insoluble food grade microcapsules by emulsification–internal gelation technique, using gelatin, pectin, alginate and chitose as immobilization material. The optical photomicroscope photograph and laser scanning confocal microscope photograph shows that the microcapsules obtained by this method has narrow size distribution (10-20 μm). Survival of Bifidobacterium bifidum in microcapsules was 109cfu•g^-1 , and the embedding ratio was 72.32%. The survival was remaining more than 108cfu•g^-1 after exposure to gastric acid and bile acid, which implies that the microcapsules expressed superior acid resistance. These microcaosules were disaggregation subtotal after 15 min in simulated intestinal juices, and the release rate was 96%. The survival was 108 cfu•g^-1 at room temperature after 1 year by classic accelerated test. The results indicate that encapsulation of Bifidobacterium bifidum in alginate microcapsules is a good approach to prolonging viability.

Abstract: The evaluation system of water environment security is improved by using fuzzy analytic hierarchy process (FAHP) in determining weights of various factors of the system. First, priority judgment matrix is FAHP uses three-scale method to construct and then fuzzy consistency judgment matrix is obtained. It overcomes many shortcomings of traditional AHP, such as the difficulty to scale when assigning weights, the need to conduct consistency testing, a large amount of calculation and low accuracy of calculation. It is effective and reasonable in the evaluation of water environment security.

Abstract: This study was designed to investigate the immunomodulating effect of polysaccharides purified from the fruit body of Pholiota adipose(PFBP). PFBP were extracted in 90°C water bath and precipitated with 90% ethanol. PFBP were orally administered at different dose to three different doses were administered to the normal group, NS group and the model group. The immunomodulating effect of PFBP was evaluated by measuring its effects on the weights of spleen and liver, delayed type hypersensitivity(DTH) response, the hemolysin level in serum and carbon expurgation level. Polysaccharide content purified from fruit body of Pholiota adipose was up to 72.88% . PFBP administered at different doses significantly improved the weights of the mouse’s spleen and live, as well as the level of carbon expurgation. Administered polysaccharides also had positive effect on DTH response and the hemolysin level in serum. These results suggest that PFBP may have immunomodulating potential for cylophosphamide.

Abstract: Higher biomass producing crops such as sunflower have already been considered as a hyperaccumulating candidate. In the present study, Cd and Pb accumulation by sunflower (Helianthus annuus L.), biomass and root exudates changes in sand cultures was analyzed during 90 days. Results demonstrated that ratios of aerial biomass/ root biomass (AW/RW) in Cd or Pb treatments declined with concentrations of Cd or Pb. Optical density (OD) of root exudates at 190 nm and 280 nm observed in Cd and Pb treatments were lower than the control. In single Cd or Pb treatments, bioconcentration factors (BCF) of Cd or Pb increased with time and decreased with concentrations, the highest Cd-BCF and Pb-BCF appeared in Cd5 (21) and Pb50 (7.95), respectively. Cd-BCF or Pb-BCF showed positive correlations with AW/RW ratios and OD values, and they were negative correlated with Cd and Pb concentrations. In the complex of Cd and Pb treatments, the highest values of BCFs were observed in Cd10+Pb100. Cd-BCF values negatively correlated with AW/RW ratios and Cd concentrations; besides, Pb-BCFs were negative with both Cd and Pb concentrations. Translocation factors (TF) fluctuated irregularly with time and concentration of metals in most treatments. Cd-TF values in single Cd treatments were positive correlated with concentrations of Cd. In the complex of Cd and Pb treatments, Cd-TF showed positive correlations with OD at 280 nm, but negative with Pb concentration. Results in this paper may supply useful information for phytoremediation of soil contaminated with cadmium and lead in situ.

Abstract: A novel process for methanol and dimethyl ether(DME) synthesis was developed, in which two methanol-synthesis fixed-bed reactors were followed by an identical one for DME synthesis with cooling sections in between. Self-made Cu-Zn-Al catalyst for methanol synthesis and DME synthesis were used in this process. Tests of catalysts showed that both of them had activity and favorable stability and wound be capable of long-term industrial operation under the conditions. The investigation of processing parameters and stability led us safely to ensure the optimal condition: T1-200oC,T2-190 °C,T3-210 °C,5Mpa,total Space Velocity-1000h-1(T1,T2,T3 defined as temperature of catalytic beds in three serial reactors). Under that the catalytic system kept stable during the investigated hours.

Abstract: Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma cell. To study its effect on the proliferation and apoptosis of different tumor cells and verify its anti-tumor non-specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay，flow cytometry，transmission electron microscopy(TEM), the biological activity of 19peptide was studied. Experiments in vitro identified that obtained 19peptide could inhibit proliferation of hela cell and hepatocarcinoma cell. It also could promote two tumor cells apoptosis. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.

Abstract: People are frequently exposed to various environmental chemicals such as organic pollutants, heavy metals, etc. Therefore, the aim of this paper is to investigate the accumulation level of organochlorines in women from Qiqihaer city in china. Of the 160 people investigated, 113 sujects were detected with different serum levels of organochlorine pesticides (range: 0.042 - 33.072 μg/L). Moreover, the results indicated that there was high serum level of accumulated organochlorines pesticides of women in Chinese, and the serum concentrations of organochlorines pesticides was associated with age.

Abstract: In wastewater treatment system, the humus soil activated sludge process is to culture microorganisms by setting the natural humus soil composite mineral filter to simulate natural soil growing conditions. After enriched and activated in the cultivation tank, the microorganisms with specific functions return to different position of activated sludge process and achieve enhanced operational performance. The project results showed the process could enhance the removal of nitrogen and phosphorus, reduce sludge production, improve sludge dewatering property and achieve odor elimination.

Abstract: The factors of species of the medium, spawn age, species of the enzyme, time of hydrolysis, hydrolysis temperature, osmotic pressure stabilizer and regeneration medium, which have important effects on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast, were discussed in this paper. These results showed that the hydrolysis condition can be described as following: Chaetomium cupreum mycelium with spawn age about 48h, the concentration of the lytic enzymes about 2% and the time of enzymatic hydrolysis 2h. The regeneration condition of the protoplast is that: Chaetomium cupreum mycelium with the spawn age 6h, the concentration of the lytic enzymes 2%, the temperature of 25°C and the time of enzymatic hydrolysis 1.5h. We can get the Chaetomium cupreum protoplast, regenerating in the RA Medium with the sorbitol, which regeneration can be over 50%.

Abstract: All aspects of measured process should be considered to minimize the negative influence then improve the GPR detection accuracy in the tunnel lining inspection. Take ChangBanPo tunnel test for example, in the case of not using measuring wheel, reducing the interpolation space improves accuracy of test data in the axial direction, and finally the positioning accuracy of the data is over 0.1m. By calculating the electromagnetic wave velocity of the end of the lining where the thickness is known, the velocity is used as the corresponding section of the lining ,which avoids the computational error by using dielectric constant and the error is controlled under the centimeter level.